摘要
如何快速、无损地检测种子活力是目前种子研究领域的热点和难点。基于种子呼吸与种子活力的关系搭建了基于可调谐半导体激光吸收高光谱(TDLAS)技术的种子活力快速无损检测系统,该系统主要由分布反馈式激光器及其控制电路、光电转换及放大电路、数据采集电路、上位机软件以及基于多次反射池结构的种子呼吸CO2浓度检测池构成。检测池的容积为1.5 L,光程为16 m,激光光源波段为2004 nm。基于朗伯比尔定律,采用波长调制吸收光谱技术利用二次谐波反演出种子呼吸过程中产生的CO2浓度。根据种子呼吸CO2浓度的大小确定种子活力的强弱,并将其与发芽出苗实验获得的活力指数进行对比。实验结果表明:CO2呼吸强度的变化量与种子活力等级指数的相关性在0.9以上,即基于TDLAS技术的种子活力快速无损检测系统能够精准、无损、高效地反映种子活力的强弱。这一研究为采用TDLAS技术进行种子活力无损检测分级提供了有益探索。
Quick and nondestructive detection of seed vigor is a popular and difficult task in the seed research field. Based on the relationship between seed respiration and seed vigor, we proposed a rapid non-destructive testing system for seed vigor based on TDLAS technique. The proposed system comprises a distributed feedback laser and its control circuit, a photoelectric conversion and amplification circuit, a data acquisition circuit, an upper computer software, and a seed breathing carbon dioxide (CO2) concentration detection pool based on a multi-reflection pool structure. The detection pool has a volume of 1.5 L, a light path of 16 m, and a laser source band of 2004 nm. Based on Lambert Beer s law, we use wavelength modulated absorption spectroscopy and second harmonic generation to invert the CO2 concentration generated during seed respiration. Seed vigor is determined according to the concentration of CO2 in seed respiration, and the vigor index obtained from the germination and seedling emergence experiment is compared and validated. The experimental results show that the correlation between the change of CO2 respiratory intensity and seed vigor grade index is greater than 0.9, i. e., the rapid non-destructive testing system for seed vigor based on TDLAS technique can accurately, nondestructively, and efficiently reflect the seed vigor grade. This study provides useful exploration in non-destructive testing and grading of seed vigor using TDLAS technique.
作者
贾良权
祁亨年
胡文军
赵光武
阚瑞峰
高璐
郑雯
许琴
Jia Liangquan;Qi Hengnian;Hu Wenjun;Zhao Guangwu;Kan Ruifeng;Gao Lu;Zheng Wen;Xu Qin(School of Information Engineering , Huzhou University , Huzhou , Zhejiang 313000, China;School of Agriculture and Food Science , Zhejiang A & F University , Hangzhou , Zhejiang 311300, China;State Key Laboratory of Applied Optics , Changchun Institute of Optics , Fine Mechanics and Physics ,Chinese Academy of Science , Changchun , J ilin 130033, China)
出处
《中国激光》
EI
CAS
CSCD
北大核心
2019年第9期289-297,共9页
Chinese Journal of Lasers
基金
国家自然科学基金(31701512,61772198)
浙江省重点研发项目(2019C02013)
湖州市自然科学基金(2017YZ03,2016YZ01)
关键词
光谱学
可调谐半导体激光吸收高光谱
无损检测
种子活力
呼吸强度
二氧化碳
spectroscopy
tunable diode laser absorption spectroscopy
nondestructive testing
seed vigor
respiratory intensity
carbon dioxide
