摘要
目的探讨急进和渐进高原对大鼠应激损伤与炎性反应的影响。方法将160只Wistar大鼠,采用随机数字表法分为北京组、兰州组、急进高原组、渐进高原组,每组40只。除北京组(海拔43.5m)外,其余大鼠空运至兰州市(海拔1520m)。随后,急进高原组继续由汽车运至玛多县(海拔4270m),渐进高原组在兰州停留3d后运至玛多县。各组分别于实验第1、2、3、5、7天取大鼠血清,采用酶联免疫吸附试验法测定血红素加氧酶-1、脑钠肽、白细胞介素-18和肿瘤坏死因子-α的水平。结果与北京、兰州组大鼠比较,急进和渐进高原组大鼠血清中4种因子的表达水平均有升高,且渐进高原组的升高程度始终低于急进高原组。结论以急进或渐进方式进入高原均会引起大鼠的应激损伤及炎性反应,但渐进高原较急进高原引起的机体损伤与炎性反应小。
Objective To investigate the effects of rapidly and gradually entering plateau on stress injury and inflammatory reaction of rats. Methods One hundred and sixty Wistar rats were randomly divided into the Beijing group,Lanzhou group,rapidly entering plateau group and gradually entering plateau group ,40 cases in each group.Except for the Beijing group(altitude 43.5 m),the rest rats were transported to Lanzhou City (altitude 1520 m) by air.Then,the rapidly entering plateau group was transported to Maduo County (altitude 4270 m) by automobile.The gradually entering plateau group was transported to Maduo County after staying in Lanzhou for 3 d.Serum was extracted in each group on 1,2,3,5,7 d of the experiment.The levels of serum HO-1,brain natriuretic peptide (BNP),interleukin-18 (IL-18) and tumor necrosis factor-α(TNF-α) were detected by ELISA. Results Compared with the Beijing group and Lanzhou group,the expression levels of the four factors in the rapidly entering plateau group and gradually entering plateau group were increased,moreover,the increase degree of the gradually entering plateau group were always lower than those in the rapidly entering plateau group. Conclusion Entering the plateau by the rapid and gradual modes all cause the stress injury and inflammatory reaction of rates,however gradually entering plateau causes small body injury and inflammatory reaction.
作者
李军茹
贾守宁
王双玺
李欣
邹小云
王慧芳
郭文斌
LI Junru;JIA Shouning;WANG Shuangxi;LI Xin;ZOU Xiaoyun;WANG Huifang;GUO Wenbin(Qinghai Provincial Hospital of Traditional Chinese Medicine,Xining,Qinghai 810000,China;Qinghai University,Xining,Qinghai 810016,China)
出处
《重庆医学》
CAS
2019年第20期3437-3439,3444,共4页
Chongqing medicine
基金
青海省科技厅研究项目(2017-ZJ-738)
关键词
急进高原
渐进高原
应激损伤
炎性反应
entering plateau rapidly
entering plateau group gradually
stress injury
inflammatory reaction
