摘要
目的检测肌萎缩侧索硬化(ALS)患者外周血中白细胞介素(IL)-8、趋化因子受体(CXCR)1、CXCR2的水平,并分析其与患者预后的关系。方法选取2011年8月至2013年12月确诊收治的ALS患者40例为研究组,选取无神经疾病及该类疾病家族遗传史的紧张性头痛患者38例为对照组。采用ELISA法检测血清IL-8水平;qRT-PCR法检测单个核细胞(PBMC)中CXCR1、CXCR2mRNA表达水平;ALS功能评定量表(ALSFRS-r)评估患者评分,计算疾病进展速率(DPR)。结果研究组血清IL-8水平[(72.69±16.47)pg/mlvs(36.32±15.81)pg/ml,t=9.940,P=0.000]、PBMC中CXCR1、CXCR2mRNA表达水平显著高于对照组。ALS患者血清IL-8水平、PBMC中CXCR1、CXCR2mRNA表达水平分别与DPR显著正相关(P均<0.01),与病程时间显著负相关(P<0.05,P<0.01);ALS患者血清IL-8水平与PBMC中CXCR1、CXCR2mRNA表达水平显著正相关(r=0.576,r=0.478,P均<0.05)。Kaplan-Meier生存分析表明,血清IL-8与PBMC中CXCR1、CXCR2mRNA高表达水平者生存率明显低于低表达水平者。结论ALS患者外周血IL-8、CXCR1、CXCR2水平显著升高,可能是ALS患者不良预后的生物标志。
Objective To detect the levels of interleukin(IL)-8,chemokine receptor(CXCR)1,and CXCR2 in peripheral blood of patients with amyotrophic lateral sclerosis(ALS) to analyze their relationships with prognosis.Methods Forty ALS patients from August 2011 to December 2013 were selected as study group,and thirty-eight patients with tension headache and without neurological diseases and family hereditary history of neurological diseases were selected as control group at the same time.ELISA was used to detect the level of IL-8 in serum,qRT-PCR method was used to detect the expression levels of CXCR1 and CXCR2 mRNAs in peripheral blood mononuclear cells(PBMC),ALS functional rating scale(ALSFRS-r) was used to assess patients′ scores and disease progression rate(DPR).Results The level of serum IL-8 [(72.69±16.47)pg/ml vs(36.32±15.81)pg/ml,t=9.94,P=0.000] and the expression levels of CXCR1 and CXCR2 mRNAs in study group were significantly higher than those in control group(all P<0.01).In ALS patients,the level of serum IL-8 and the expression levels of CXCR1 and CXCR2 mRNAs in PBMC were positively correlated with DPR(all P<0.01),respectively, and negatively correlated with duration of disease(P<0.05,P<0.01);the serum IL-8 level was significantly positively correlated with the expression levels of CXCR1 and CXCR2 mRNAs(r=0.576,r=0.478,all P<0.05).Kaplan-Meier survival analysis showed that the survival rates of ALS patients with high levels of serum IL-8,CXCR1 and CXCR2 in PBMC was significantly lower than that of ALS patients with low levels of above indicators.Conclusion For patients with ALS,the levels of IL-8,CXCR1 and CXCR2 increase significantly,which may be the biomarkers of poor prognosis.
出处
《中国临床研究》
CAS
2019年第10期1327-1330,共4页
Chinese Journal of Clinical Research
基金
国家自然科学基金资助项目(81571256)~~
