摘要
AIM: To investigate the expression of p28/gankyringene and its role in the carcinogenetic process of humanhepatocellular carcinoma (HCC).METHODS: 64 specimens of HCC and para-carcinomatissues, 22 specimens of non-tumor liver tissues (7normal, 15 cirrhosis), 10 specimens of normal humantissues and 5 hepatoma cell lines were studied for theexpression of p28/gankyrin by Northern blot. Theexpression of p28/gankyrin protein was detectedimmunohistochemically by using the specificpolyclonal antibody. RESULTS: Northern blot analysis indicated that theexpression of p28/gankyrin mRNA was intensivelydistributed in brain and heart, weakly in lung, spleenand muscle, undetectable in digestive system includingliver, pancreas, stomach, small and large intestines.p28/gankyrin mRNA was absent in normal liver, weaklydetected in liver cirrhosis and in 18 of 64 para-carcinoma liver tissues. In contrast, the expression ofp28/gankyrin mRNA was intensively detected in ali 5hepatoma cell lines tested, markedly increased in 57of 64 and moderately increased in 5 of 64 HCC samples.In comparison with liver cirrhosis and para-carcinomaliver tissues, the average expression of p28/gankyrin mRNA in HCC was increased 3.6- (2.901+0.507 vs 0.805 + 0.252, P＜0.05) and 5.2-fold (2.901 +0.507 vs 0.557+0.203, p＜0.01), respectively, Inaddition, p28/gankyrin mRNA expression level washigher in HCC with portal vein tumor thrombus andmicroscopic hepatic vein involvement (P--0.021 andP=-0.047, respectively). The overexpression of p28/gankyrin protein in HCC was targeted in hepatic tumorcells, not in bile duct cells and other interstitial cells.CONCLUSION: Overexpression of p28/gankyrin in HCCplays an important role and contributes to themetastasis potential in the process of carcinogenesis.p28/gankyrin may become a specific biological tissuemarker for the pathological diagnosis of HCC.
AIM:To investigate the expression of p28/gankyrin gene and its role in the carcinogenetic process of human hepatocellular carcinoma(HCC). METHODS:64 specimens of HCC and para-carcinoma tissues,22 specimens of non-tumor liver tissues(7 normal,15 cirrhosis),10 specimens of normal human tissues and 5 hepatoma cell lines were studied for the expression of p28/gankyrin by Northern blot.The expression of p28/gankyrin protein was detected immunohistochemically by using the specific polyclonal antibody. RESULTS:Northern blot analysis indicated that the expression of p28/gankyrin mRNA was intensively distributed in brain and heart,weakly in lung,spleen and muscle,undetectable in digestive system including liver,pancreas,stomach,small and large intestines. p28/gankyrin mRNA was absent in normal liver,weakly detected in liver cirrhosis and in 18 of 64 para- carcinoma liver tissues.In contrast,the expression of p28/gankyrin mRNA was intensively detected in all 5 hepatoma cell lines tested,markedly increased in 57 of 64 and moderately increased in 5 of 64 HCC samples. In comparison with liver cirrhosis and para-carcinoma liver tissues,the average expression of p28/ gankyrin mRNA in HCC was increased 3.6-(2.901± 0.507 vs 0.805±0.252,P<0.05)and 5.2-fold(2.901± 0.507 vs 0.557±0.203,p<0.01),respectively.In addition,p28/gankyrin mRNA expression level was higher in HCC with portal vein tumor thrombus and microscopic hepatic vein involvement(P=0.021 and P=0.047,respectively).The overexpression of p28/ gankyrin protein in HCC was targeted in hepatic tumor cells,not in bile duct cells and other interstitial cells. CONCLUSION:Overexpression of p28/gankyrin in HCC plays an important role and contributes to the metastasis potential in the process of carcinogenesis. p28/gankyrin may become a specific biological tissue marker for the pathological diagnosis of HCC.
基金
the Chinese National Distinguished Young Scholar Awards,No.39825114
Chinese National Key Project of Basic Research,No.G1998051210
the Key Project of the Chinese National Natural Science Foundation,No.39830080.
