摘要
目的 :探讨牛珀至宝微丸对局灶性脑缺血再灌注后神经干细胞Nestin表达的影响。方法 :采用大脑中动脉线栓法造成局灶性脑缺血再灌注模型 ,应用免疫组织化学技术检测神经上皮干细胞巢蛋白 (Nestin)的表达 ,观察牛珀至宝微丸对脑缺血后神经上皮干细胞巢蛋白的影响。结果 :脑缺血再灌注后 ,牛珀至宝微丸组缺血侧室管膜、室管膜下区、皮层和纹状体Nestin阳性细胞数显著多于缺血对照组 (P <0 0 5 ) ,且神经病学评分明显低于缺血对照组 (P <0 0 5 )。结论 :牛珀至宝微丸能持续上调脑缺血再灌注后Nestin的表达 ,这可能是其治疗缺血性脑血管病的机理之一。
Objective]To investigate the effect of Niu po zhi bao pellet on expression of nestin following focal cerebral ischemic reperfusion. [Methods] To make the model of focal cerebral ischemia by inserting nylon thread into the internal carotid artery in rat and dectect expression of Nestin by immunohistochemical method, the effects of Niu po zhi bao pellet on expression of nestin following focal cerebral ischemia reperfusion were observed. [Results] After cerebral ischemic reperfusion, the neurologic dificit score in Niu po zhi bao pellet group was significantly lower than those of control group ( P <0 05); the number of Nestin positive cell in subventricular zone (SVZ),cerebral cortex and striatum in ischemic side in Niu po zhi bao pellet group were markedly more than those of ischemic control group ( P <0 05)[conclusien] Niu po zhi bao pellet can alleviate the neurological symptoms and continually induced up regulation of expression of Nestin, it is one of the protective mechanisms of Niu po zhi bao pellet on cerebral ischemia.
出处
《中国中医基础医学杂志》
CAS
CSCD
2002年第10期17-21,共5页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家自然科学基金资助项目 (No.3 9970 914 )
