摘要
目的:探讨苦参碱(matrine,MAT)抑制胃癌MGC-803细胞增殖和迁移的分子机制。方法:不同质量浓度的MAT处理MGC-803细胞后,应用MTT法检测MGC-803细胞的增殖活力,平板克隆形成实验检测MGC-803细胞的克隆形成能力,划痕愈合实验和Transwell小室法检测MGC-803细胞的横向及纵向迁移能力,FCM法检测MGC-803细胞的细胞周期分布,蛋白质印迹法检测MGC-803细胞中上皮-间质转化(epithelialmesenchymal transition,EMT)相关标志蛋白和基质金属蛋白酶(matrix metalloproteinase,MMP)家族成员MMP2和MMP9蛋白的表达水平。结果:MAT可显著抑制MGC-803细胞增殖(P<0.01)、克隆形成(P<0.05)以及横向和纵向迁移(P值均<0.01),诱导MGC-803细胞周期阻滞于G0/G1期(P<0.01)。MAT显著下调了MGC-803细胞中Vimentin(P<0.05)、Snail(P<0.01)蛋白以及MMP2(P<0.01)、MMP9(P<0.01)蛋白的表达水平,上调E-cadherin(P<0.05)蛋白表达水平。结论:MAT可有效抑制胃癌MGC-803细胞增殖和迁移,阻滞细胞周期于G0/G1期,其机制可能与MAT调控EMT相关蛋白表达有关。
Objective:To investigate the molecular mechanisms of matrine(MAT)inhibiting the proliferation and migration of gastric cancer MGC-803 cells.Methods:MGC-803 cells were treated with different concentrations of MAT.MTT assay was used to test the proliferation activity of MGC-803 cells.The colony-forming assay was used to detect the colony formation ability of MGC-803 cells.Wound healing assay and Transwell chamber assay were used to detect the lateral and vertical migration abilities of MGC-803 cells,respectively.The cell cycle distribution was detected by FCM.The protein expression levels of epithelial-mesenchymal transition(EMT)markers and matrix metalloproteinase(MMP)family members(MMP2 and MMP9)in MGC-803 cells were detected by Western blotting.Results:MAT significantly inhibited the proliferation(P<0.01),colony formation(P<0.05)and lateral and vertical migration(both P<0.01)of MGC-803 cells,and blocked the cell cycle at G0/G1 phase(P<0.01).MAT significantly decreased the expression levels of Vimentin(P<0.05),Snail(P<0.01),MMP2(P<0.01)and MMP9(P<0.01)proteins,while the expression level of E-cadherin protein was up-regulated(P<0.05).Conclusion:MAT may effectively suppress the proliferation and migration of gastric cancer MGC-803 cells,and block cell cycle at G0/G1 phase.The mechanism may be related to the regulation of EMT-related protein expression.
作者
王莹
焦璐
乔丹
林贞花
李柱虎
朴英实
WANG Ying;JIAO Lu;QIAO Dan;LIN Zhenhua;LI Zhuhu;PIAO Yingshi(Cancer Research Center,Yanbian University,Yanji 133000,Jilin Province,China;Key Laboratory of Jilin Provincial Science and Technology Department,Yanji 133000,Jilin Province,China;Department of Pathology,Zhumadian City Center Hospital,Zhumadian 463000,Henan Province,China)
出处
《肿瘤》
CAS
CSCD
北大核心
2019年第7期534-541,共8页
Tumor
关键词
胃肿瘤
细胞增殖
细胞周期
细胞迁移分析
苦参碱
Stomach neoplasms
Cell proliferation
Cell cycle
Cell migration assays
Matrine
