摘要
采用激光剥蚀-电感耦合等离子体质谱技术(Laser ablation-inductively coupled plasma-mass spectrometry,LA-ICP-MS),建立了定量分析单细胞中银纳米颗粒(AgNP)的方法。利用喷墨打印机制备出与细胞基体相似的皮升级液滴,作为单细胞定量标准,再利用LA-ICP-MS定量分析暴露AgNP的小鼠巨噬细胞。结果表明,95个单细胞中AgNP含量为19.1~1682 fg,呈对数正态分布,平均值为(166±188)fg,与酸消解分析得到的细胞群体AgNP平均含量((175±2)fg)相符。LA-ICP-MS在单细胞分析方面具有巨大潜力,有望进一步应用于单细胞水平的金属纳米颗粒的生物效应和安全性研究。
In recent years,single cell analysis has become one of hot topics in analytical chemistry.In this work,a method was developed for quantitative analysis of silver nanoparticles(AgNP)in single cells by laser ablation-inductively coupled plasma-mass spectrometry(LA-ICP-MS).An inkjet printer was used to produce droplets of silver standards,which were similar to the matrixes of single cells.After exposed to AgNP,95 single cells were quantitatively analyzed by LA-ICP-MS using the droplet standards.The contents of AgNP in single cells showed a lognormal distribution,ranging from 19.1 fg to 1682 fg Ag per cell with an average of(166±188)fg Ag per cell,which was in good agreement with the average from cell population((175±2)fg Ag per cell).LA-ICP-MS had great potential in single cell analysis and provided a new tool for studying biological effects of metal nanoparticles at a single cell level.
作者
付东旭
郑令娜
刘金辉
汪冰
竺云
王萌
丰伟悦
FU Dong-Xu;ZHENG Ling-Na;LIU Jin-Hui;WANG Bing;ZHU Yun;WANG Meng;FENG Wei-Yue(College of Physics and Materials Science,Tianjin Normal University,Tianjin 300387,China;Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100049,China;College of Sciences,Northeastern University,Shenyang 110819,China)
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2019年第9期1390-1394,共5页
Chinese Journal of Analytical Chemistry
基金
国家重点研发计划(Nos.2016YFA0201604,2018YFB0703504)
国家自然科学基金项目(Nos.U1432241,11505194,11575209)资助~~
关键词
银纳米颗粒
激光剥蚀-电感耦合等离子体质谱
单细胞分析
定量分析
Sliver nanoparticles
Laser ablation-inductively coupled plasma-mass spectrometry
Single cell analysis
Quantitative analysis
