摘要
目的运用近红外光谱(NIRS)法,建立防风中升麻素苷和5-O-甲基维斯阿米醇苷质量分数的快速测定方法。方法采用HPLC测定防风药材中2种色原酮的质量分数,并采用NIRS结合偏最小二乘法(PLS),建立2种色原酮成分质量分数间的定量校正模型。结果防风中升麻素苷和5-O-甲基维斯阿米醇苷的NIRS预测值与HPLC实测值之间具有良好的线性关系,其模型的校正相关系数(Rc)分别为0.975 8、0.992 3;预测相关系数(Rp)分别为0.904 0、0.907 3;校正标准偏差(RMSEC)分别为0.028、0.011 9;预测标准偏差(RMSEP)分别为0.055 6、0.041 3。结论该方法可快速、准确地对防风中2种色原酮成分进行定量分析。
Objective To establish a rapid method for the determination of prim-O-glucosylcimifugin and 4′-O-beta-glucopyranosyl-5-O-methylvisamminol in Radix Saposhnikoviae by NIRS.Methods HPLC was used to determine two chromones in Radix Saposhnikoviae.Two chromones quantitative calibration model was established by combining NIRS with partial least squares(PLS).Results There was a good linear relationship between the NIRS predicted values of prim-O-glucosylcimifugin,5-O-methyl-vismi-tol and the HPLC measured values in Radix Saposhnikoviae.The corrected correlation coefficients of the calibration models were 0.975 8,0.992 3 and the predictive correlation coefficient were 0.904 0,0.907 3.The calibration set cross-validation root mean square error(RMSEC)was 0.028,0.011 9,and the root mean square error of prediction(RMSEP)were 0.055 6,0.041 3.Conclusion The NIRS method can quantitatively analyze prim-O-gluco-sylcimifugin and 5-O-methyl-vismitol in Radix Saposhnikoviae.
作者
卢泳
孙冬梅
王洛临
李智勇
李树民
李洁环
冯健英
陈雪婷
徐文杰
LU Yong;SUN Dongmei;WANG Luolin;LI Zhiyong;LI Shumin;LI Jiehuan;FENG Jianying;CHEN Xueting;XU Wenjie(The Fifth Clinical Medicine College,Guangzhou University of Chinese Medicine,Guangzhou 510095,China;Guangdong Province Engineering Technology Research Institute of T.C.M/Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine,Guangzhou 510095,China)
出处
《广东药科大学学报》
CAS
2019年第4期517-522,共6页
Journal of Guangdong Pharmaceutical University
基金
广东省名优中成药二次开发项目(20174006)
广东省科技计划项目“创新中药研发平台建设”(2017A070701017)
广东省中医药科技创新平台建设项目(粤中医函[2017]340号)
关键词
近红外光谱法
防风
色原酮
快速测定
定量模型
near-infrared spectroscopy
the Radix Saposhnikoviae
chromone
rapid determination
quantitative model
