摘要
金莲花(Trollius chinensis)是毛茛科的一种药用及观赏植物,采用Illumina HiSeq2500高通量技术对其全基因组DNA测序,建立126 bp DNA小片段文库,以大兔葵(Megaleranthis saniculifolia)叶绿体基因组为参考,通过BLASTN比对提取后用CLC Genomics Workbench软件组装得到金莲花叶绿体全基因组序列,并对其特征进行分析。结果表明,金莲花叶绿体基因组全长160 191 bp,具有典型的被子植物叶绿体基因组环状四分体结构,两个反向互补重复序列(inverted repeat, IRA和IRB)的长度均为26 632 bp,大单拷贝区(large single copy, LSC)和小单拷贝区(small single copy, SSC)的长度分别为88 522 bp和18 405 bp;注释得到131个基因,包括85个蛋白质编码基因,36个tRNA基因,8个rRNA基因和2个假基因,其中18个基因包括一个或两个内含子;共检测到231个简单重复序列(short simple repeat, SSR)。此叶绿体基因组已在GenBank注册,序列号为KX752098。本研究为金莲花叶绿体分子标记研究提供了理论依据,有助于促进该物种分子育种、进化分析和系统发育的研究。
Trollius chinensis is a medical and ornamental plant in Ranunculaceae. Total genomic DNA sequences was sequenced using Illumina Hi Seq 2 500 high-throughput technology, then the small fragments(126 bp) of DNA libraries were constructed. The whole chloroplast genome sequences of Trollius chinensis were extracted by BLASTN software according to complete chloroplast(cp) genome of Megaleranthis saniculifolia, and assembled into the cp genome of Trollius chinensis by using CLC Genomics Workbench software. Then analyzed its basic characteristics. The results showed that the length of Trollius chinensis cp genome was 160 191 bp with a typical cyclic tetrad structure of chloroplast genome in angiosperms, containing a pair of inverted repeats(IRA and IRB) of26 632 bp that were separated by a large single-copy region(LSC) and a small single-copy region(SSC) of 88 522 bp and 18 405 bp, respectively;A total of 131 predicted genes, including 85 protein-coding genes, 8 ribosomal RNA genes, 36 transfer RNA genes and 2 misc-feature genes were identified, among which 18 genes including one or two introns;Additionally, 231 short simple repeats(SSRs) loci of cp genome were detected. The cp genome of Trollius chinensis was submitted to GenBank with the accession number of KX752098. The study research provided a theoretical basis for the study of the molecular marker of the chloroplast of the Trollius, which could help to promote the molecular breeding, evolution and phylogenetic analysis of this species.
作者
雷慧
朱思锦
赵艳
段霞霞
Lei Hui;Zhu Sijin;Zhao Yan;Duan Xiaxia(Biology Department,Xinzhou Teachers University,Xinzhou,034000)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2019年第8期3595-3604,共10页
Genomics and Applied Biology
基金
忻州师范学院院级青年基金项目(QN201536)资助
关键词
金莲花
叶绿体基因组
高通量测序
特征分析
Trollius chinensis
Chloroplast genome
High-throughput sequencing
Characteristic analysis
