摘要
为探讨甘草炮制雷公藤后对DSS诱导的溃疡性结肠炎(UC)小鼠的抗炎作用及对肠道免疫系统的调节作用。该研究建立DSS诱导的UC小鼠动物模型,以美沙拉嗪(Mes)为阳性药;通过小鼠疾病活动指数(DAI)、结肠长度、结肠组织病理学评分等分析甘草炮制雷公藤低(PT1)、高(PT2)剂量给药后的药效作用;通过检测小鼠血清中的TNF-α和IL-6细胞因子表达水平,小鼠结肠的免疫组化CD3+T及Fox P3+Treg染色等分析甘草炮制雷公藤对UC小鼠的抗炎及免疫调节作用;研究还通过小鼠肝脏组织HE染色及血清ALT,AST水平分析各剂量甘草炮制雷公藤的肝毒性情况。结果显示,PT1组、PT2组给药后小鼠结肠炎症状减轻,炎症细胞浸润减少,炎症因子表达降低,且分别与模型组相比,差异具有统计学意义(P<0. 05);甘草炮制雷公藤高、低剂量组肝脏HE染色及ALT,AST水平与正常组相比无显著差异。研究结果显示,甘草炮制雷公藤对UC小鼠具有抗炎及免疫调节作用,同时该剂量未呈现明显的肝毒性。
The aim of this paper was to investigate the anti-inflammatory effect of Tripterygium wilfordii processed with licorice on DSS-induced ulcerative colitis(UC)mice and its regulation on intestinal immune system.In this study,a DSS-induced animal model of UC mice was established,with mesalazine(Mes)as a positive drug.The pharmacodynamic effects of low(PT1)and high(PT2)doses of T.wilfordii processed with licorice were analyzed by disease activity index(DAI),colon length and colon histopathological score in mice.By detecting the expression levels of TNF-αand IL-6 cytokines in the serum of mice,immunohistochemical CD3+T and FoxP3+Treg staining in the colon of mice,the anti-inflammatory and immunoregulatory effects of T.wilfordii processed with licorice on UC mice were analyzed.The hepatotoxicity of each dose of T.wilfordii processed with licorice was also analyzed by HE staining in liver tissue of mice and ALT and AST levels in serum.The results showed that the colitis symptoms of the mice in the PT1 group and the PT2 group were alleviated,the inflammatory cell infiltration was reduced.And the expression of inflammatory factors was decreased,the difference was statistically significant compared with the model group(P<0.05).The HE staining and ALT and AST levels in the high dose group and low dose group were not significantly different from those in the normal group.The results showed that T.wilfordii pro-cessed with licorice has the anti-inflammatory and immunomodulatory effects on UC mice,and the dose did not show significant hepato-toxicity.
作者
吴昊
章从恩
于小红
马光朝
王伽伯
马致洁
赵奎君
WU Hao;ZHANG Cong-en;YU Xiao-hong;MA Guang-chao;WANG Jia-bo;MA Zhi-jie;ZHAO Kui-jun(Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;the Fifth Medical Center of PLA General Hospital,China Military Institure of Chinese Medicine,Beijing 100039,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第16期3435-3440,共6页
China Journal of Chinese Materia Medica
基金
首都卫生发展科研专项(首发2016-4-2024)
北京市中医药科技项目(QN2014-18)
关键词
甘草炮制雷公藤
溃疡性结肠炎
抗炎
免疫调节
Tripterygium wilfordii processed with licorice
ulcerative colitis
anti-inflammatory
immune regulation
