摘要
目的观察异鼠李素(Isorhamnetin)对结肠癌细胞株SW48衰老的影响。方法通过AnnexinⅤ/PI流式细胞术检测细胞凋亡,筛选适宜的异鼠李素剂量,通过β-GAL染色检测异鼠李素对细胞衰老的影响,分别通过CCK8法及BrdU/PI双掺入法测定细胞增殖及DNA合成能力,继而通过免疫印迹法(Western blot)检测SW48细胞中衰老相关蛋白的变化。结果在SW48中加入不同浓度的异鼠李素,观察到当异鼠李素的浓度≤40 nM时,SW48细胞的凋亡水平没有明显的改变。因此后续实验中选择10 nM、20 nM及40 nM3种药物剂量。10 nM、20 nM及40 nM的异鼠李素作用下,SW48细胞衰老增加,细胞活性减低,细胞中DNA合成能力即细胞增殖能力下降,并且衰老及增殖相关蛋白如p53、p21等发生明显变化。结论异鼠李素可能通过促进细胞衰老抑制肿瘤细胞的增殖和生长。
Objective To observe the effect of Isorhamnetin on the cell senescence of colon cancer cell line SW48.Methods Different doses of Isorhamnetin were incubated with the SW48 cells,then the effect of Isorhamnetin on the apoptosis of SW48 cells was detected by Annexin V/PI assay,and the change of cell senescence was observed byβ-GAL.The cell proliferation was measured by CCK8 assay and BrdU incorporation assay.Finally,the expression of cell senescence related proteins were detected by Western blot.Results The apoptosis rates of SW48 was remain similar when the concentration of Isorhamnetin was under 40 nM,thus 10 nM,20 nM and 40 nM Isorhamnetin were used in the further experiments.The cell senescence of SW48 cells was elevated after Isorhamnetin incubation for 48 h,with cell proliferation reduced,and the expression of cell senescence related proteins was enhanced obviously.Conclusion Isorhamnetin may inhibit the proliferation and growth of cancer cells by promoting cell senescence.
作者
朱栋良
严海东
黎尉浩
尹小平
ZHU Dongliang;YAN Haidong;LI Weihao(Department of Gastrointestinal SurgeryⅡWard,Xianning Cental Hospital,Xianning 437100,China)
出处
《临床外科杂志》
2019年第8期684-687,共4页
Journal of Clinical Surgery
关键词
异鼠李素
结肠癌
衰老
增殖
Isorhamnetin
colon cancer
senescence
proliferation
