摘要
目的探讨尿液中白细胞介素-8(IL-8)和中性粒细胞明胶酶相关脂质运载蛋白(NGAL)的水平变化与尿路感染及早期肾脏损伤的关系。方法一共将6周岁以下儿童120例纳入研究,其中确诊为尿路感染的患儿80例作为尿路感染组,包括40例初次确诊和40例治疗后复发的尿路感染患者,40例未发生急慢性尿路感染的健康儿童。采用发光免疫分析仪分别检测入组儿童尿液标本中IL-8和NAGL的浓度,分析两者在不同临床分组中的表达变化,对结果进行统计学分析。结果与对照组比较,NAGL、IL-8在尿路感染组中均显著升高(P<0.05),并且初次诊断组的水平明显高于复发组(P<0.05);同时,伴有合并症的尿路感染患儿NAGL水平显著高于单纯尿路感染的患儿(P<0.05)。结论IL-8可作为尿路感染的早期诊断候选指标,NAGL可作为尿路感染伴早期肾脏损伤的特征性指标,为快速诊断儿童尿路感染提供实验依据,并用于开发快速、简单、低成本的儿童尿路感染诊断方法和临床监测方法。
Objective To explore the diagnostic values of urine IL-8 and neutrophil gelatinase-associated lipocalin(NAGL)in urinary tract infection(UTI)and to provide evidence for kidney damage in the early stage.Methods A total of 120 cases of children under 6 years of age from the pediatric outpatient department were enrolled in this study,80 children of which were confirmatory diagnosed with urinary tract infection including 40 initially diagnosed and 40 recurrence cases,and 40 healthy children without acute or chronic urinary tract infection.The concentration of urine IL-8 and NGAL among different groups were detected by using chemical immunofluorescent.All of the data were statistically analyzed.Results The concentrations of IL-8 in the initial diagnosing group were higher than those in the control group(P<0.05).The expression of NAGL was significantly different between initial diagnosing groups and the control group(P<0.05),meanwhile,the concentration of NAGL in UTI complication group was higher than those with simple UTI but no complications(P<0.05).Conclusion IL-8 can be used as a candidate marker for early diagnosis of urinary tract infection,and NAGL can be used as a characteristic marker for urinary tract infection with early renal injury.In order to provide experimental basis for rapid diagnosis of urinary tract infections in children,it is helpful to develop rapid,simple and low-cost methods for diagnosis and clinical monitoring of urinary tract infections in children.
作者
余抒
刘跃平
府伟灵
YU Shu;LIU Yueping;FU Weiling(Department of Clinical Laboratory,Stomatological Hospital Affiliated to the Fourth Military Medical University,State Key Laboratory of Military Stomatology/National Center for Clinical Medicine of Stomatological Diseases/Shaanxi Center for Clinical Medicine of Stomatological Diseases,Xi′an,Shaanxi 710032,China;Department of Clinical Laboratory,the 911 Hospital of the Joint Logistics Support Force of the Chinese People′s Liberation Army,Xiangyang,Hubei 441000,China;Department of Clinical Laboratory,the First Hospital Affiliated to Army Medical University,Chonqing 400038,China)
出处
《国际检验医学杂志》
CAS
2019年第18期2191-2194,共4页
International Journal of Laboratory Medicine
基金
国家自然科学基金(81702096)
陕西省重点研发计划(2018SF-128)
