摘要
目的探讨TNF-α、CD40基因多态性与乙肝肝硬化的相关性研究。方法运用PCR-RFLP结合测序的方法,测定150例健康对照者与150例乙肝肝硬化患者血细胞中的TNF-α-308G/A、CD40-rsl883832、CD40-rs4810485的基因多态性,并确定其基因型的分布。结果乙型肝炎后肝硬化患者和健康对照者相比较,肝硬化患者的TNF-α-308G/A的GA基因型明显高于健康者,差异有统计学意义(P<0.05)。肝硬化患者的CD40-rsl883832的CT、TT基因型明显高于健康者,差异有统计学意义(P<0.05)。TNF-α-308G/A的AA、GG基因型、CD40-rsl883832的CC基因型、CD40-rs4810485的基因型差异无统计学意义(P>0.05)。结论TNF-α、CD40基因多态性与乙肝肝硬化的遗传易感性有关。
Objective To investigate the relationship between TNF-α,CD40 gene polymorphism and hepatitis B cirrhosis.Methods The polymorphism of TNF-α-308G/A,CD40-rsl883832,CD40-rs4810485 in the blood cells of 150 healthy controls and 150 patients with hepatitis B cirrhosis were determined,and the genotype distribution was determined.Results The GA genotype of TNF-α-308G/A in patients with post-hepatitis B cirrhosis was significantly higher than that in healthy controls(P<0.05).The CT,TT genotypes of CD40-rsl883832 in cirrhotic patients were significantly higher than those in healthy subjects(P<0.05).The AA,GG genotype of TNF-α-308G/A and the CC genotype of CD40-rsl883832 were significantly higher than those of healthy subjects(P<0.05).There was no significant difference in genotype of CD40-rs4810485(P>0.05).Conclusion the polymorphism of TNF-αand CD40 gene is associated with the susceptibility to hepatitis B cirrhosis.
作者
吴应冬
周宝勤
谢群
周安琪
WU Yingdong;ZHOU Baoqin;XIE Qun;ZHOU Anqi(Department of Clinical Laboratory,People′s Hospital of Haian City,Haian,Jiangsu 226600,China)
出处
《国际检验医学杂志》
CAS
2019年第18期2215-2218,共4页
International Journal of Laboratory Medicine
