摘要
采用过氧化氢刺激lager型啤酒酵母,Tadpoling法筛选细胞活力恢复较快突变菌,根据各菌株线粒体膜电位、胞内氧自由基(ROS)水平筛选获得4株活性较高菌株。比较突变菌与原始菌传代发酵性能、胞内ROS、活力指标及每一代细胞死亡率水平,最终获得一株抗氧化能力提高且活力稳定性较高啤酒酵母菌株。分析验证突变菌线粒体DNA修复相关基因MHR1发现,该基因发生部分碱基突变。
In this study,hydrogen peroxide was used to mutate lager yeast,and Tadpoling method was used to screen mutant strains with rapid cell vitality recovery.Four strains with higher activities were screened according to mitochondrial membrane potential and intracellular reactive oxygen species(ROS)level of each strain.The serial fermentation performance,intracellular ROS,vitality index and mortality levels of each generation of the mutants and the original strain were compared.Finally,a brewer's yeast strain with higher antioxidant capacity and higher vitality was obtained.Mutations were found in the MHR1 gene which was related to mitochondrial DNA repair.
作者
李磊
王金晶
郑梅莹
郑飞云
钮成拓
刘春凤
李崎
LI Lei;WANG Jinjing;ZHENG Meiying;ZHENG Feiyun;NIU Chengtuo;LIU Chunfeng;LI Qi(The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi Jiangsu 214122,China;Laboratory of Brewing Science and Technology,Jiangnan University,Wuxi Jiangsu 214122,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2019年第8期40-49,共10页
Journal of Northeast Agricultural University
基金
国家自然科学基金项目(31771963,31571942)
