摘要
【目的】研究重组人乳铁蛋白对断奶仔猪生长性能及血液生化指标的影响。【方法】选取28日龄,体重均匀的断奶仔猪180头,试验设6个处理,每个处理3次重复,每个重复10头仔猪(公母各半)。A处理饲喂基础日粮,作为对照;B处理添加抗生素,在基础日粮中添加1 g/kg 10%的杆菌肽锌+200 mg/kg 10%的抗敌素;C、D、E、F处理在基础日粮基础上分别添加160、320、480、640 mg/kg重组人乳铁蛋白。试验为期28 d。【结果】(1)试验末重与日均增重B、C、D、E、F处理均显著高于A处理,日均采食量B、E处理显著高于A处理,料重比B、D、E、F处理显著低于A处理,腹泻指数B、D、E、F处理显著低于A、C处理。(2)E处理血清白蛋白、总蛋白含量显著高于A处理,B、E处理血清尿素氮含量显著低于A处理。(3)血清总抗氧化能力,C、D、E、F处理显著高于A处理。D、F处理血清谷胱甘肽过氧化物酶活性显著高于A处理,E处理血清总超氧化物歧化酶活性显著高于A、B处理,MDA浓度A处理显著高于C、D、E、F处理。【结论】断奶仔猪日粮中添加重组人乳铁蛋白能提高断奶仔猪生长性能、改善血清生化指标及提高抗氧化能力。综合试验结果,重组人乳铁蛋白添加量为480 mg/kg的效果最好。
【Objective】The experiment was conducted to investigate the effects of recombinant human lactoferrin(rHL)on the growth performance and serum biochemical index of weaned piglets.【Method】One hundred and eighty 28-dayold weaned piglets(with uniform weight),were assigned randomly to 6 treatment groups and there were 3 replicates in each group with 10 piglets per replicate(5 males and 5 females).The control group A were fed with a basal diet,and group B piglets were fed a basal diet supplemented with antibiotic(1 g/kg 10% bacitracin zinc+200 mg/kg 10% colistin).Groups C,D,E and F were fed with basal diets supplemented with 160,320,480 and 640 mg/kg rHL,respectively.The experiment lasted for 28 days.【Result】The results showed as follows:(1)the final weight and average daily gain(ADG)of groups B,C,D,E and F were higher than those of group A(P<0.05).The daily intake of groups B and E was significantly higher than that of group A(P<0.05).The ratio of weight gain to intake of groups B,D,E and F was significantly lower than that of groups A(P<0.05).The diarrhea index of groups B,D,E and F was lower than that of groups A and C(P<0.05).(2)The concentrations of serum albumin and TP of group E were higher than that those of group A(P<0.05).The urea nitrogen of groups B and E was higher than that of group A(P<0.05).(3)The T-AOC level of groups C,D,E and F was higher than that of group A(P<0.05).The GSH-Px activity of groups D and F was higher than that of group A(P<0.05).The T-SOD level of group E was higher than that of groups A and B(P<0.05).The MDA content of group A was lower than that of groups C,D,E and F(P<0.05)【.Conclusion】The above data showed the supplemented rHL could improve the growth performance,serum biochemical index and antioxidant ability of weaned piglets.The optimum level of rHL was 480 mg/kg based on the test results.
作者
梁耀文
舒会友
黄新全
陈玲
刘燊
张辉华
LIANG Yaowen;SHU Huiyou;HUANG Xinquan;CHEN Ling;LIU Shen;ZHANG Huihua(College of Life Science and Engineering,Foshan University,Foshan 528225,China;Guangzhou Guanglilai Biotechnology Co.,Ltd.,Guangzhou 510080,China;Animal Inspection Station of Longyuanba Town,Quannan County,Jiangxi Province,Quannan 341800,China;Bureau of Agricultural and Rural of Quannan County,Jiangxi Province,Quannan 341800,China)
出处
《广东农业科学》
CAS
2019年第9期128-134,共7页
Guangdong Agricultural Sciences
基金
国家重点研发计划项目(2018YFD0501202-3)
广东省自然科学基金(2016A030313378)
广东省科技计划项目(2015A020209195)
广东省教育厅高校科研项目(2017GCZX006)
关键词
重组乳铁蛋白
断奶仔猪
生产性能
腹泻率
生化指标
抗氧化指标
recombinant human lactoferrin
weaned piglet
growth performance
diarrhea rate
biochemical index
antioxidant index
