摘要
目的研究整合素α5在子宫颈癌中的表达及其与患者临床病理特征的关系。方法应用免疫组织化学染色法检测青岛市市立医院2014年1月至2017年12月60例子宫颈癌石蜡包埋组织及20例因子宫良性病变行全子宫切除术的正常子宫颈石蜡包埋组织中整合素α5蛋白的表达水平。应用实时荧光定量聚合酶链反应(RT-PCR)检测青岛市市立医院2018年1月至7月20例新鲜子宫颈癌组织及20例良性子宫病变的正常子宫颈组织中整合素α5 mRNA的表达水平。分析整合素α5的表达与子宫颈癌患者临床病理特征的关系。结果整合素α5蛋白在子宫颈癌及正常子宫颈组织中的阳性表达率分别为63.3%(38/60)、35.0%(7/20),差异有统计学意义(χ2=4.893,P<0.05)。整合素α5 mRNA在子宫颈癌中相对于正常子宫颈组织的表达量(2-△△Ct)为1.6±0.4(t=5.529,P<0.01)。整合素α5蛋白的阳性表达与子宫颈癌的淋巴结转移有关(Z=-2.636,P=0.008)。结论整合素α5在子宫颈癌组织中高表达,且与淋巴结转移有关,有望成为子宫颈癌治疗的新靶点。
Objective To investigate the expression of integrinα5 in cervical cancer,and to explore its relationship with clinicopathological characteristics of patients with cervical cancer.Methods Immunohistochemistry was used to detect the expression of integrinα5 in cervical cancer tissues of 60 cases and normal cervical paraffin-embeded tissues of 20 cases of benign uterine lesions undergoing hysterectomy from Qingdao Municipal Hospital between January 2014 and December 2017.Real-time quantitative polymerase chain reaction(RT-PCR)was used to detect the mRNA expression level of integrinα5 in 20 fresh cervical cancer tissues and 20 normal cervical tissues collected from benign cervical lesions in Qingdao Municipal Hospital between January 2018 and July 2018.The relationship between the expression of integrinα5 and the clinicopathological characteristics of patients with cervical cancer was analyzed.Results The positive expression rate of integrinα5 protein in cervical cancer and normal cervical tissues was 63.3%(38/60),35.0%(7/20),respectively,and the difference was statistically significant(χ2=4.893,P<0.05).The expression of integrinα5 mRNA in cervical cancer was 1.6±0.4 times as high as that in normal cervical tissues(t=5.529,P<0.01).The positive expression of integrinα5 protein was associated with lymph node metastasis in cervical cancer patients(Z=-2.636,P=0.008).Conclusion The high expression of integrinα5 is related to lymph node metastasis of cervical cancer,and integrinα5 may be a new potential target for treatment of cervical cancer.
作者
杜维民
张裕民
谭雪莹
徐晓娜
张萍
Du Weimin;Zhang Yumin;Tan Xueying;Xu Xiaona;Zhang Ping(Department of Gynecology,Qingdao Municipal Hospital Affiliated to Qingdao University,Qingdao 266011,China;Heze Institute for Food and Drug Control of Shandong Province,Heze 274000,China;Key Laboratory of Cell Transplantation of Health Ministry,Qingdao Municipal Hospital Affiliated to Qingdao University,Qingdao 266011,China;Department of Central Laboratory,Qingdao Municipal Hospital Affiliated to Qingdao University,Qingdao 266011,China)
出处
《肿瘤研究与临床》
CAS
2019年第10期658-661,共4页
Cancer Research and Clinic
基金
青岛市民生科技计划(14-2-3-20-nsh)。
关键词
宫颈肿瘤
整合素Α5
淋巴结转移
Uterine cervical neoplasms
Integrin alpha 5
Lymph node metastasis
