摘要
研究阿魏酸对高产酪胺的粪肠球菌XL-M66和屎肠球菌XL-M76生长、基因表达以及产酪胺的影响。利用反转录实时荧光定量聚合酶链式反应技术分析2株菌在阿魏酸作用下的酪氨酸脱羧途径相关基因表达情况,并使用高效液相色谱法检测2株肠球菌培养48 h期间酪胺积累量。结果表明:未添加酪氨酸底物时,阿魏酸对酪氨酸脱羧酶(tyrosine decarboxylase,tyrDC)和酪氨酸/酪胺透性酶(tyrosine/tyramine permease,tyrP)基因的转录影响不大(P>0.05),但能促进酪氨酰-tRNA合成酶(tyrosyl-tRNA synthetase,tyrS)基因的转录(P<0.05)。反之存在酪氨酸时,阿魏酸对tyrS基因表达的影响不大(P>0.05),却能显著抑制tyrDC和tyrP基因的表达(P<0.05)。同时,阿魏酸能显著抑制粪肠球菌XL-M66和屎肠球菌XL-M76的生长(P<0.05),最终使得酪胺产量分别降低27.0%和19.9%。
This study determined the effects of ferulic acid on the growth,gene expression and tyramine accumulation of Enterococcus faecalis XL-M66 and E.faecium XL-M76.Reverse transcription quantitative polymerase chain reaction(RT-qPCR)was used to analyze the expression of tyramine synthesis-related genes in the tyrosine decarboxylation pathway in E.faecalis XL-M66 and E.faecium XL-M76.Additionally,the accumulation of tyramine during 48 h culture was monitored by high performance liquid chromatography(HPLC).Results showed that ferulic acid had little effect on the transcription of the tyrosine decarboxylase(tyrDC)and tyrosine/tyramine permease(tyrP)genes(P>0.05)in the absence of the substrate tyrosine,but promoted the transcription of the tyrosyl-tRNA synthetase(tyrS)gene(P<0.05).On the contrary,in the presence of tyrosine,ferulic acid had little effect on the expression of the tyrS gene(P>0.05),but it could significantly(P<0.05)inhibit the expression of the tyrDC and tyrP genes.Moreover,ferulic acid significantly(P<0.05)inhibited the growth of E.faecalis XL-M66 and E.faecium XL-M76 and reduced tyramine production by 27.0%and 19.9%,respectively.
作者
薛林林
王远
李彬彬
王庆玲
卢士玲
XUE Linlin;WANG Yuan;LI Binbin;WANG Qingling;LU Shiling(Food College,Shihezi University,Shihezi 832003,China;Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第22期33-38,共6页
Food Science
基金
国家自然科学基金面上项目(31360392)
关键词
阿魏酸
屎肠球菌
粪肠球菌
酪胺
酪氨酸脱羧酶
反转录实时荧光定量聚合酶链式反应
ferulic acids
Enterococcus faecalis
Enterococcus faecium
tyramine
tyrosine decarboxylase
reverse transcription quantitative polymerase chain reaction
