摘要
从7种新疆冷水鱼肠道中分离筛选低温益生乳酸菌,根据16S rRNA基因序列结合多重聚合酶链式反应指纹分型技术对菌株遗传结构差异进行分析,结果显示其中15株隶属于肉杆菌属(Carnobacterium),包括两个已知C.divergens(10株)和C.maltaromaticum(4株)及未鉴定的菌株Carnobacterium spp.编号(1株)。采用牛津杯法初筛显示8个菌株代谢物对3种致病菌均有不同程度的抑制作用,对单核细胞性李斯特菌的抑菌效果最明显。8个菌株发酵上清液经排除有机酸、过氧化氢及蛋白酶实验处理,发现除菌株BS-JYC-3经胰蛋白酶处理抑菌活性未丧失外,其余菌株经酶解实验抑菌能力完全丧失,表明发酵上清液中抑菌物质可能为类细菌素。药敏实验显示所有菌株对氨苄西林、米诺环素和氯霉素表现敏感,对庆大霉素、四环素、利福平及替考拉宁表现中度敏感。
Psychrotolerant probiotic lactic acid bacteria were isolated from the guts of 7 cold-water fish species in Xinjiang and were analyzed for genetic structure differences according to the sequences of their 16S rRNA gene by rep-PCR fingerprint typing technology.The results showed that 15 strains of Carnobacterium were obtained,including 4 strains of C.maltaromaticum,10 strains of C.divergens and 1 unidentified strain of Carnobacterium spp..By the Oxford cup method,it was found that the metabolites of 8 of these strains had different inhibitory effects on three pathogens,and the strongest antibacterial effect was observed against Listeria monocytogenes.The fermentation supernatants of the 8 strains were subjected to exclusion of organic acids and hydrogen peroxide or protease treatment.After protease treatment,all the supernatants except BS-JYC-3 completely lost their antibacterial activity,indicating that the bacteriostatic substance in the fermentation supernatants may be a bacteriocin.Drug susceptibility tests showed that all of the 8 strains were very sensitive to ampicillin,minocycline and chloramphenicol,and moderately sensitive to gentamicin,tetracycline,rifampicin and teicoplanin.
作者
魏小晶
赵志霞
罗宝龙
张瑞蕊
张艳
倪永清
周红
WEI Xiaojing;ZHAO Zhixia;LUO Baolong;ZHANG Ruirui;ZHANG Yan;NI Yongqing;ZHOU Hong(Food College,Shihezi University,Shihezi 832003,China;College of Life Sciences,Shihezi University,Shihezi 832003,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第22期68-74,共7页
Food Science
基金
国家自然科学基金地区科学基金项目(31360001)
关键词
冷水鱼肠道
肉杆菌
指纹图谱
抑菌活性
抗生素敏感性
cold-water fish intestine
Carnobacterium
fingerprint
inhibitory activity
antibiotics resistance
