摘要
制备醋酸纤维素-聚丙烯复合膜,并将转谷氨酰胺酶(transglutaminase,MTG)固定在膜上,制得了固定化MTG酶膜,测得其酶活为17.6 U/g。然后将酶膜固定在不锈钢网框上,并将其悬挂在烧杯中,用来处理米糠废水中的蛋白质。利用单因素试验,分别考察物料温度、物料pH值、转子转速以及酶膜面积对蛋白质聚合率的影响,并在单因素试验基础上,利用响应面试验优化,得到最佳聚合条件为物料温度43℃、pH 6.6、转子转速124 r/min、酶膜面积与底物含量比例为80∶1(cm^2/g)、反应时间1.8 h,蛋白质聚合率为70%。固定化酶膜在使用5次后,相对酶活力仍保持在74.3%以上,为连续聚合米糠废水中的蛋白质以及保留营养物质提供理论基础。
In this study,cellulose acetate-polypropylene composite membrane was prepared,and microbial transglutaminase(MTG)was immobilized onto the membrane,yielding an MTG-immobilized membrane,whose enzymatic activity was determined to be 17.6 U/g.Then the enzyme-loaded membrane was fixed on a stainless steel mesh frame and suspended in a beaker in order to use it to treat protein in rice bran wastewater.The effects of material temperature,material pH,rotor speed and enzyme-loaded membrane area on protein polymerization rate were investigated by one-factor-at-a-time method.Furthermore,using response surface optimization,the optimum polymerization conditions were obtained as follows:material temperature 43℃,pH 6.6,rotor speed 124 r/min,ratio between enzyme-loaded membrane area and substrate amount 80:1(cm2/g),and reaction time 1.8 h.Under these conditions,protein polymerization rate was 70%.The enzymeimmobilized membrane remained above 74.3%of the original activity after its fifth repeated use.This study provides a theoretical basis for the continuous polymerization of proteins in rice bran wastewater and the retention of nutrients.
作者
裴兴武
汪鸿
高子雯
袁泰增
高明
姚凯
潘明喆
于殿宇
PEI Xingwu;WANG Hong;GAO Ziwen;YUAN Taizeng;GAO Ming;YAO Kai;PAN Mingzhe;YU Dianyu(College of Food Science,Northeast Agricultural University,Harbin 150030,China;Hubei Tianxing Grain and Oil Co.Ltd.,Suizhou 441300,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第22期110-117,共8页
Food Science
基金
“十三五”国家重点研发计划重点专项(2018YFD0401101)
关键词
转谷氨酰胺酶
复合酶膜
米糠废水
蛋白质聚合率
重复使用
transglutaminase
enzyme-loaded composite membrane
rice bran wastewater
protein polymerization rate
repeated use
