摘要
采用响应面优化真空耦合超声波提取龙牙楤木皂苷的工艺条件,并对比研究真空耦合超声波提取技术、常压超声辅助技术、溶剂提取技术对龙牙楤木皂苷抗氧化活性和抑菌活性的影响。结果表明,真空耦合超声波提取技术最佳工艺为乙醇溶液体积分数80%、料液比1∶25 g/mL、超声温度58℃、超声时间50 min、超声功率270 W、真空度0.08 MPa,在此条件下,龙牙楤木皂苷得率为4.18%,该方法与常压超声辅助技术、溶剂提取技术相比,可提高皂苷得率并明显缩短提取时间。抗氧化活性试验表明,真空耦合超声波技术提取的龙牙楤木皂苷体外抗氧化活性显著(P<0.05)高于常压超声辅助技术和溶剂提取技术制备的龙牙楤木皂苷,其中真空耦合超声波技术制备的龙牙楤木皂苷对DPPH自由基的IC50值为0.344 mg/mL,对超氧阴离子由基的IC50值为1.367 mg/mL,总还原能力OD值最大为0.893。抑菌活性试验表明,龙牙楤木皂苷对大肠杆菌、金黄色葡萄球菌有抑制效果,对枯草芽孢杆菌无抑制效果,其中真空耦合超声波提取龙牙楤木皂苷抑菌效果最好,对大肠杆菌最小抑菌浓度为2.5 mg/mL,对金黄色葡萄球菌最小抑菌浓度为10 mg/mL。
The reaction conditions were optimized by vacuum-coupled ultrasonic extraction of the saponins of the saponin from Aralia elata,and the anti-oxidation and antibacterial activities of the saponins of the saponins were studied by vacuum coupled ultrasonic extraction,atmospheric pressure ultrasonic assisted and solvent extraction techniques.influences.Results showed that,the optimum conditions were 80%ethanol,material-solvent ratio 1∶25 g/mL,ultrasonic temperature 58℃,duration 50 min,ultrasonic power 270 W,vacuum level 0.08 MPa,yield of Aralia elata saponin was 4.18%.This technology could significantly increased the yield and shorted the duration compared with the other two technologies.The antioxidant test showed that the antioxidant activity of Aralia elata saponin extracted by ultrasonic coupled vacuum technology was closed to that of Vitamin C,and significantly higher than those of saponins extracted by atmospheric pressure ultrasonic extraction and solvent extraction.The IC50 values of ultrasonic coupled vacuum technology extracted Aralia elata saponin were 0.344 mg/mL to DPPH radical,1.367 mg/mL to hydroxyl radical,and the maximum total reduction capacity OD value was 0.893.Antibacterial activity test showed that Aralia elata saponin had inhibitory effects on Escherichia coli and Staphylococcus aureus,but no inhibitory effect on Bacillus subtilis.The ultrasonic coupled vacuum technology extracted Aralia elata saponin showed strongest inhibitory effects,while solvent extracted one showed the weakest effect.The minimum inhibitory concentrations of ultrasonic coupled vacuum technology extracted Aralia elata saponin were 2.5 mg/mL to Escherichia coli and 10 mg/mL to Staphylococcus aureus.
作者
李德海
朱晓冉
王路
王振宇
赵楠楠
龚金华
LI De-hai;ZHU Xiao-ran;WANG Lu;WANG Zhen-yu;ZHAO Nan-nan;GONG Jin-hua(College of Forestry,Northeast Forest University,Harbin 150040,China;College of Chemistry and Chemical Engineering,Harbin Institute of Technology,Harbin 150090,China;Faculty of Liberal Arts and Social Sciences,Education University of Hong Kong,Hong Kong 999077,China)
出处
《食品工业科技》
CAS
北大核心
2019年第22期24-33,39,共11页
Science and Technology of Food Industry
基金
“十三五”国家重点研发计划项目(2016YFC0500307-07)
关键词
龙牙楤木皂苷
真空耦合超声波
提取
响应面法
抗氧化活性
抑菌活性
Aralia elata saponin
ultrasonic coupled vacuum technology
extraction
surface response method
antioxidant activity
antibacterial activity
