摘要
为探讨微生物复合菌系对纤维素的协同降解效果,从西藏农田土壤和长沙稻田腐叶堆积物中筛选出6株高温纤维素降解菌,构建纤维素高效降解复合菌系M6,分析复合菌系M6对堆肥物料的温度、pH值、总有机碳含量、总氮含量、碳氮比、总养分含量和种子发芽指数的影响。结果表明,复合菌系M6处理具有较好的CMC-Na酶活性和滤纸酶活性,在50℃下发酵25 d时,接种复合菌系M6的油菜秸秆降解率可达16.4%;复合菌系M6处理较其他处理具有较高的堆肥温度及较长的高温时间,在发酵的第3天迅速达到温度峰值(63.7℃),其高温期可持续8 d;复合菌系M6处理的堆肥物料pH值呈弱碱性;堆肥末期,复合菌系M6处理总有机碳含量由50.44%下降至32.77%,总氮含量由1.79%升高到2.28%,碳氮比由28.18下降至14.19,总养分含量为13.27%;复合菌系M6处理的种子发芽指数始终高于其他处理,堆肥末期种子发芽指数为107%,复合菌系M6处理的堆肥具有较好的腐熟度及品质。
In order to investigate the synergistic degradation effect of microbial complex strains on cellulose,six high-temperature cellulose degrading bacteria were screened from the farmland soil in Tibet and rice field leaf decay deposits in Changsha,and a high efficient cellulose degradation composite strains M6 was constructed.The effects of composite strains M6 on the temperature,pH value,total organic carbon content,total nitrogen content,carbon nitrogen ratio,total nutrient content and seed germination index of compost materials were analyzed.The results showed that,composite strains M6 treatmeat had better CMC-Na enzyme activity and filter paper enzyme activity,When fermented 25 d at 50℃,the degradation rate of rape straw inoculated with composite strains M6 could reach 16.4%.The composite strains M6 treatment had higher compost temperature and longer high-temperature time than other treatments,the peak temperature reached 63.7℃on the 3rd day of fermentation,and the high-temperature period lasted for 8 days;The pH value of compost materials inoculated with composite strains M6 treatment showed weak alkalinity;at the end of composting,the content of total organic carbon in composite strains M6 treatment decreased from 50.44%to 32.77%,and the content of total nitrogen increased from 1.79%to 2.28%,the C/N decreased from 28.18 to 14.19,and the total nutrient content was 13.27%.The GI value of the composite strains M6 treatment was always higher than other treatments,the GI value was 107%at the end of the compost,the composting treated with composite strains M6 had better permeability and quality.
作者
刘青海
潘虎
朱兆静
田云
王翀
达娃卓玛
卢向阳
白军平
LIU Qinghai;PAN Hu;ZHU Zhaojing;TIAN Yun;WANG Chong;DAWA Zhuoma;LU Xiangyang;BAI Junping(Institute of Agricultural Product Quality Standard and Testing Research,Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet 850032,China;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,china)
出处
《河南农业科学》
北大核心
2019年第12期56-62,共7页
Journal of Henan Agricultural Sciences
基金
西藏自治区财政专项(XZNKY-2018-C-026)
国家“十二五”科技支撑计划项目(2012BAD15B04-5)
西藏自治区自然科学基金(XZ2017ZRG-42)
西藏自治区科技计划项目
长沙市科技计划项目(kq1706025)
湖南农业大学“双一流”建设项目(SYL201802002)
湖南农业大学青年基金项目(16QN12)
关键词
纤维素降解
CMC-Na酶活性
滤纸酶活性
复合菌系
堆肥效果
西藏
协同作用
高温
Cellulose degradation
CMC-Na enzyme activity
Filter paper enzyme activity
Composite strains
Compost effect
Tibet
Synergies
High-temperature
