摘要
目的探讨罗格列酮(rosiglitazone,RGZ)对脂多糖(lipopolysaccharide LPS)诱导的新生大鼠急性肺损伤气道炎症的影响及其机制。方法清洁级SD新生大鼠随机分为对照组、LPS组及罗格列酮干预组。LPS组鼻腔中滴入LPS的剂量为4 mg/kg,对照组给予等量生理盐水,罗格列酮干预组鼻腔中滴入LPS(剂量同前)1 h后腹腔内注射罗格列酮(2 mg/kg);对照组和LPS组鼻腔滴入等量LPS或生理盐水1 h后腹腔注射等量生理盐水。各组新生鼠分别于6、12、24 h被处死,然后评估肺组织的病理评分、湿/干质量比值(W/D)、支气管肺泡灌洗液(BALF)中多形核白细胞(PMN)计数,并检测BALF中的IL-17水平。结果与对照组相比,不同时间点LPS组的肺组织炎症病理评分、W/D、BALF中的PMN值及IL-17水平均升高(P<0.05),而罗格列酮干预组肺组织炎症病理评分、W/D、BALF中的PMN值及IL-17水平在不同时间点均较LPS组降低(P<0.05)。结论罗格列酮可显著减轻LPS诱导的新生鼠肺部炎症,其机制可能与其抑制IL-17分泌相关。
To investigate the effects of rosiglitazone on airway inflammation induced by lipopolysaccharide in neonatal rats with acute lung injury, newborn SD rats were recruited and randomly divided into control, LPS and RGZ intervention groups. The LPS group was received LPS(4 mg/kg) via nasal cavity and the control group was given equal amount of saline. The rosiglitazone intervention group was given LPS(4 mg/kg) via nasal cavity and then received intraperitoneal injection of RGZ at the dose of 2 mg/kg 1 hour later. For control group or LPS group, the same amount of saline or LPS was dropped into the nasal cavity and then the same amount of saline was intraperitoneally injected. The rats in each group were sacrificed at 6, 12 and 24 hours respectively after the last administration, with the aim of evaluating the pathological score of lung tissue, wet/dry weight ratio(W/D),polymorphonuclear leukocyte(PMN) count, and IL-17 level in bronchoalveolar lavage fluid(BALF). Compared with the control group, LPS group demonstrated higher levels of lung inflammatory score, W/D ratio, PMN ratio and IL-17 level at different time points(P<0.05), while rosiglitazone intervention could reverse the changes(P<0.05). In conclusion, rosiglitazone can significantly reduce LPS-induced pulmonary inflammation, and the mechanism might be related to the inhibition of IL-17 secretion.
作者
罗艳
李清香
杨洁
雷贤明
曹云涛
LUO;Yan LI Qingxiang;YANG Jei;LEI Xianming;CAO Yuntao(Department of Neonatology,Affiliated Hospital of Zunyi Medical University,Zunyi 563003,China)
出处
《免疫学杂志》
CAS
CSCD
北大核心
2019年第12期1074-1080,共7页
Immunological Journal
基金
遵义医科大学硕士启动基金（院字2012(02)号）
关键词
肺损伤
脂多糖
SD新生大鼠
Lung injury
Lipopolysaccharide
Sprague-Dawley neonatal rat
