摘要
以五加科的掌叶梁王茶和八角金盘未展开幼叶叶尖为试材,采用常规压片法制片,研究了优化染色体制片条件和核型分析,以期为供试物种的育种工作开展提供依据。结果表明:掌叶梁王茶的最佳预处液为0.002 mol·L-18-羟基喹啉和0.04%放线菌酮混合液,八角金盘则为0.001 mol·L-18-羟基喹啉。2个物种其余的最佳制片方法相同,具体为09:00-09:30取样、V无水乙醇:V冰醋酸=3:1卡诺固定液处理12~24 h、V无水乙醇:V浓盐酸=1:1解离液处理13 min、改良苯酚品红染色液处理10 min。掌叶梁王茶染色体核型为2n=48=28m+18sm+2st,不对称系数61%,2B核型;八角金盘为2n=48=34m+14sm,不对称系数60%,2A核型。与已报道的五加科植物核型进行比较,2个物种进化程度不高,研究结果丰富了对供试物种遗传组成的认知。
The unexpanded leaf tips of Metapanax dalavayi and Fatsia japonica,two landscape plants in Araliaceae,were used as materials in this study.The chromosome staining factors were optimized and karyotype characteristics were also investigated.The results showed that the best pretreatment solution of Metapanax dalavayiis 0.002 mol·L-18-HQ+0.04%actidione,whereas 0.001 mol·L-18-HQ was the best for Fatsia japonica.The rest optimization conditions of this two testing plants were sample at 09:00-09:30 a.m.,fixing in Carnoy′s solution(one part glacal acetic acid and three parts 95%ethanol)for 12-24 hours.The fixed material was macerated in dissociation liquid(the volume ratio of anhydrous alcohol and hydrochloric acid was 1 to 1)for 13 minutes,and then stained for 10 minutes with carbolfuchsin solution.The karyotype of Metapanax dalavayi and Fatsia japonicawere 2B(2n=48=28m+18sm+2st)and 2A(2n=48=34m+14sm),and the karyotype asymmetry index(As.K)were 61%and 60%,respectively.Compared the results with those from the reported plants in Araliaceae,these two testing plants were relatively primitive species.These results would enrich the awareness of genetic composition of Metapanax dalavayi and Fatsia japonica,and provide cytological evidence for the breeding of these two testing plants.
作者
赵雁
杜康华
李宛宣
邵艳东
张杰
ZHAO Yan;DU Kanghua;LI Wanxuan;SHAO Yandong;ZHANG Jie(Collage of Landscape and Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201)
出处
《北方园艺》
CAS
北大核心
2019年第22期83-89,共7页
Northern Horticulture
基金
国家自然科学基金资助项目(31660058)
云南农业大学学生科技创新创业行动基金资助项目(2017-92-210)
关键词
掌叶梁王茶
八角金盘
染色体
制片优化
核型分析
Metapanax dalavayi
Fatsia japonica
chromosome
preparation optimization
karyotype analysis
