摘要
目的:测定接骨木(耐盐碱品种、天然红1号品种、青州15号品种)根皮、茎、叶、果实的活性成分含量,并对其化学成分的量进行化学模式识别。方法:采用紫外分光光度法(UV)测定多糖、黄酮、酚酸、三萜含量;采用p H示差法测定花青素含量;采用高效液相色谱法(HPLC)测定熊果酸、齐墩果酸、没食子酸、芦丁、槲皮素、原儿茶酸、莫诺苷含量。并采用化学模式识别的方法对3个品种接骨木的根皮、茎、叶、果实中的含量进行分析。结果:通过主成分分析中的F3,F6综合评分,进一步评价不同样品质量,结果发现,根皮和叶在综合排名中较靠前,其结果与其定量测定结果基本吻合。天然红1号品种根皮多糖和莫诺苷质量分数最高,分别为161.09,8.33 mg·g^-1;黄酮、芦丁、槲皮素在天然红1号品种叶中质量分数最高,分别为71.93,4.09,3.57 mg·g^-1;三萜在天然红1号品种果实中质量分数最高,为47.84 mg·g^-1;酚酸和花青素在耐盐碱品种果实中质量分数最高,分别为11.25,94.32 mg·g^-1;耐盐碱品种茎中齐墩果酸质量分数最高,为1.41 mg·g^-1;耐盐碱品种叶中熊果酸、没食子酸质量分数最高,分别为2.12,0.34 mg·g^-1;青州15号品种根皮中原儿茶酸质量分数最高,为0.12 mg·g^-1。结论:天然红1号品种含量较其他两个品种高,质量好。
Objective:To determine the content of active ingredients in root bark,stems,leaves and fruits of three varieties of Sambucus williamsii(Saline-toleratedvariety,Natural Red No.1 variety,Qingzhou No.15 variety),and identify the chemical patterns of the chemical components.Method:The content of polysaccharides,flavonoids,phenolic acids and triterpene were determined by UV spectrophotometry(UV)colorimetry.The content of anthocyanin was determined by pH differential method.Ursolic acid,oleanolic acid,quercetin,protocatechuic acid,morroniside content were determined by high performance liquid chromatography(HPLC).The root bark,stems,leaves and fruits of three varieties were analyzed by chemical pattern recognition.Result:The quality of different samples was further evaluated by F3 and F6 comprehensive scores in principal component analysis.It was found that the root bark and leaves were higher in the comprehensive ranking,and the results were basically consistent with the quantitative results.The contents of polysaccharide and morroniside in Natural Red No.1 variety root bark were the highest,which were 161.09 mg·g^-1 and 8.33 mg·g^-1,respectively.Flavonoids,rutin and quercetin were the highest in the Natural Red No.1 variety leaf,which were71.93,4.09 and 3.57 mg·g^-1,respectively.Triterpene was the highest in Natural Red No.1 variety fruit,which was 47.84 mg·g^-1.Phenolic acid and anthocyanin were the highest in Saline-tolerated variety fruits,which were 11.25 mg·g^-1 and 94.32 mg·g^-1,respectively.The Saline-tolerated variety stem had the highest oleanolic acid content,which was 1.41 mg·g^-1.Saline-tolerated variety leaf had the highest contents of ursolic acid and gallic acid,which were 2.12 and 0.34 mg·g^-1,respectively.The highest content of protocatechuic acid in Qingzhou No.15 variety root bark,which was 0.12 mg·g^-1.Conclusion:The content of Natural Red No.1 variety is higher than that of the other two varieties,with a good quality.
作者
孙丹丹
姚俊修
王刚
和焕香
林宪臣
郭庆梅
吴德军
SUN Dan-dan;YAO Jun-xiu;WANG Gang;HE Huan-xiang;LIN Xian-chen;GUO Qing-mei;WU De-jun(Shandong University of Traditional Chinese Medicine,Ji'nan 250355,China;Shandong Academy of Forestry,Ji'nan 250014,China;Pingyin County Forestry Bureau,Ji'nan 250400,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第24期124-130,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
山东省农业良种工程项目(2016LZGC017)
山东省研究生教育创新计划项目(SDYY15110)
关键词
接骨木
耐盐碱品种
天然红1号品种
青州15号品种
根皮
茎
叶
果实
Sambucus williamsii
Saline-toleratedvariety
Natural Red No.1variety
Qingzhou No.15 variety
root bark
stems
leaves
fruits
