摘要
蕲蛇是一种传统名贵中药材,具有免疫调节、抗肿瘤、抗炎、镇痛等药理作用,临床主要用于治疗类风湿性关节炎、强直性脊柱炎、干燥综合征和肿瘤等疾病。蕲蛇毒腺中分泌的蛇毒蛋白具有凝血、抗癌、抗栓、改善微循环和神经毒性等功能。为挖掘蕲蛇中蛇毒蛋白功能基因,利用基因工程技术在体外实现大量蛇毒蛋白的表达和纯化,该研究利用高通量测序平台对蕲蛇毒腺进行了转录组测序,组装获得了32862条unigene,其中26589条被公共数据库成功注释,2695条被注释到62个转录因子家族中,并且预测出5920个SSR位点。对分泌性磷脂酶A2和C型凝集素进行序列分析和同源建模,结果显示分泌性磷脂酶A2是同源二聚体,每个单体中包含5个保守的氨基酸构成了酶的催化活性中心;C型凝集素则有一个保守的糖基化结合位点,可以特异结合N-乙酰-D-葡萄糖胺。该研究对蕲蛇毒腺进行转录组测序,为蕲蛇毒腺中蛇毒蛋白功能的研究奠定了基础,将促进蕲蛇基因资源的开发和利用。
Agkistrodon acutus is a traditional Chinese herb medicine which has immunological regulation,anti-tumor,anti-inflammatory and analgesic effects,which is mainly used for the treatment of rheumatoid arthritis,ankylosing spondylitis,sjogren’s syndrome and tumors. In order to excavate more important functional genes from A. acutus,the transcriptome of the venom gland was sequenced by the Illumina Hi Seq 4000,and 32 862 unigenes were assembled. Among them,26 589 unigenes were mapped to least one public database. 2 695 unigenes were annotated and assigned to 62 TF families,and 5 920 SSR loci were identified. The majority of mapped unigenes was from Protobothrops mucrosquamatus in the NR database,which revealed their closest homology. Three secretory phospholipase A2 with different amino acid sequences showed similar spatial structures and all had well-conserved active sites. The 3 D structural models of C-type lectin showed conserved glycosylation binding sites( Asn45). This study will lay the foundation for the further study of the function of snake venom protein,and promoting the development and utilization of genome resources from A. acutus.
作者
张声祥
施圆圆
单春苗
王涛
汪振兴
汪胜松
吴家文
ZHANG Sheng-xiang;SHI Yuan-yuan;SHAN Chun-miao;WANG Tao;WANG Zhen-xing;WANG Sheng-song;WU Jia-wen(Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine,Hefei 230012,China;Anhui Institute of Qi'men Snake Medicine,Huangshang 245600,China;Key Laboratory of Xinan Medicine,Ministry of Education,Anhui University of Chinese Medicine,Hefei 230038,China;Synergetic Innovation,Center of Anhui Authentic Chinese Medicine Quality Improvement,Hefei 230012,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第22期4820-4829,共10页
China Journal of Chinese Materia Medica
基金
中央本级重大增减支项目(2060302)
安徽高校自然科学研究重大项目(KJ2018ZD028)
安徽省重点研究与开发计划项目(1704a0802146)
黄山市科技计划项目(2017KN-21)
安徽省自然科学基金项目(1608085MH177)
