摘要
Recently,phage display technology has been announced as the recipient of Nobel Prize in Chemistry 2018.Phage display technique allows high affinity target-binding peptides to be selected from a complex mixture pool of billions of displayed peptides on phage in a combinatorial library and could be further enriched through the biopanning process;proving to be a powerful technique in the screening of peptide with high affinity and selectivity.In this review,we will first discuss the modifications in phage display techniques used to isolate various cancer-specific ligands by in situ,in vitro,in vivo,and ex vivo screening methods.We will then discuss prominent examples of solid tumor targeting-peptides;namely peptide targeting tumor vasculature,tumor microenvironment(TME)and overexpressed receptors on cancer cells identified through phage display screening.We will also discuss the current challenges and future outlook for targeting peptidebased therapeutics in the clinics.
基金
This work was supported by grants from the National Key Research and Development Program of China(2016YFC1302300)
the Natural Science Foundation of China(Grant Nos.81720108029,81621004,81490750,81874226 and 81803020)
Guangdong Science and Technology Department(2016B030229004)
Guangzhou Science Technology and Innovation Commission(201803040015)
The research is partly supported by Fountain-Valley Life Sciences Fund of University of Chinese Academy of Sciences Education Foun datio n.
