摘要
氨氮污染严重影响对虾健康养殖,为了筛选适用于对虾养殖高效降解氨氮微生态菌,从实验室前期分离的25株菌株中,通过氨氮降解检测筛选得到氨氮降解效果较强的4株菌株FS007、FS008、FS017、FS025,其中FS017菌株为最佳氨氮降解菌株,对其培养24 h后进行形态学鉴定:菌落呈白色,表面湿润,边缘光滑呈圆形,中间凸起,有黏液;细胞革兰氏染色呈阳性,杆状,聚合在一起呈短链状或念珠状排列,芽孢椭圆形,中生。生理生化试验:酪素、葡萄糖、明胶、木糖、甘露醇、阿拉伯糖、淀粉、柠檬酸、硝酸盐还原、甲基红试验、过氧化氢酶、VP反应、需氧性、NaCl(2%~12%)等都表现为阳性,而产气、果糖、丙酸盐H 2S等表现为阴性。通过16S rDNA分子鉴定,确定该菌为贝莱斯芽孢杆菌Bacillus velezensis。
The pollution of ammonia nitrogen has seriously affected the healthy aquaculture of shrimp.In order to screen the microecological bacteria suitable for the efficient degradation of ammonia nitrogen in shrimp aquaculture,four strains including FS007,FS008,FS017 and FS025 with the strong effect of ammonia nitrogen degradation were obtained from 25 strains isolated from the early stage of the laboratory through the detection of ammonia nitrogen degradation.Among them,FS017 was the best strain with the degradation of ammonia nitrogen.After 24 h of culture,its morphological identification was carried out:The bacterial colony was white,the surface was wetting,the edge was smooth and round,and there was mucilage in the middle,while the cells were positive for gram staining,rod-shaped and arranged in short chain or Beadlike chain together with elliptic and central endospores.And the physiological and biochemical tests were also carried out:the results of casein,glucose,gelatin,xylose,mannitol,arabinose,starch,citric acid,nitrate reduction,methyl red test,catalase,VP reaction,aerobism and NaCl(2%-12%)were all positive,while the results of aerogenesis,fructose and propionate H 2S were negative.Therefore,the bacterium was identified as Bacillus velezensis by 16S rDNA molecular identification.
作者
雷阳
张倩
陈钰
范玲玉
郑毅
LEI Yang;ZHANG qian;CHEN Yu;FAN Ling-yu;ZHENG Yi(College of Life Science,Fujian Normal University,Fuzhou,Fujian 350117,China)
出处
《福建农业科技》
2019年第10期16-20,共5页
Fujian Agricultural Science and Technology
基金
福州国家海洋局十三五海洋经济创新发展示范项目(FZHJ03)
关键词
对虾养殖氨氮降解
菌种鉴定
筛选
贝莱斯芽孢杆菌
Degradation of ammonia nitrogen in shrimp aquaculture
Identification of strains
Screening
Bacillus velezensis
