摘要
为了研究皱纹盘鲍、西氏鲍、绿鲍和杂色鲍等4种鲍的核型特征,实验利用荧光 原位杂交(fluorescence in situ hybridization, FISH)技术比较定位了上述4种鲍的45S rDNA位 点。皱纹盘鲍中约83%的中期细胞均检出2对45S rDNA位点,分别位于13号和16号染色体 的长臂端部。西氏鲍中约75%的中期细胞均检出3对45S rDNA位点,分别位于6号染色体 短臂端部、14号和17号染色体长臂端部。绿鲍中约85%的中期细胞均检出3对45S rDNA位点, 分别位于4号、6号和8号染色体长臂的端部。杂色鲍中约65%的中期细胞均检出3对45S rDNA, 位点,分别位于3号、4号和12号染色体短臂的端部。此外,4种鲍均有少数中期相的45S rDNA位点数高于众数,这提示,除了明确的45S rDNA位点外,4种鲍可能均有若干个不 稳定的45S rDNA位点。实验结果丰富了鲍细胞遗传学研究资料,同时为鲍的遗传育种研 究提供了基础数据。
Abalone is not only precious seafood in China, but also important mariculture shellfish. In recent years,the genetic breeding research of abalone has made rapid progress, but the research data of cytogenetic analysis arestill lacking. Therefore, this study used the fluorescence in situ hybridization (FISH) to compare the distribution of45S rDNA clusters in Haliotis discus hannai, H. gigantea, H. fulgens, and H. diversicolor. In H. discus hannai,83% of the metaphase had 2 pairs of 45S rDNA sites, located at terminal of the long arm ends of chromosomes13th and 16th, respectively. In H. gigantea, about 75% of the metaphase had 3 pairs of 45S rDNA sites, located atthe terminal of the short arm of chromosome 6th, and at the terminal of the long arm of chromosomes 14th and17th, respectively. In H. fulgens, about 85% of the metaphase cells detected three pairs of 45S rDNA sites, locatedat the terminal of the long arms of chromosomes 4th, 6th, and 8th. In H. diversicolor, about 65% of the metaphasehad 3 pairs of 45S rDNA sites, located at the terminal of the short arms of chromosomes 3th, 4th, and 12th. Inaddition to the main mode, there were other low-frequency modes in all the 4 species of abalone, suggesting thatthese abalone may have several unstable 45S rDNA sites besides the unambiguous sites. The number and thelocation of 45S rDNA loci in abalone showed a high level of intraspecific variation in general. These findings mayenrich the cytogenetic research data of abalone and provide basic data for the further studies of genetic breeding inabalone.
作者
张健鹏
王铜毅
骆轩
游伟伟
柯才焕
蔡明夷
ZHANG Jianpeng;WANG Tongyi;LUO Xuan;YOU Weiwei;KE Caihuan;CAI Mingyi(Key Laboratory of Healthy Mariculture for the East China Sea,Ministry of Agriculture and Rural Affairs,Fisheries College,Jimei University,Xiamen 361021,China;Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources,College of Ocean and Earth Sciences,Xiamen University,Xiamen 361102,China)
出处
《水产学报》
CAS
CSCD
北大核心
2019年第12期2459-2467,共9页
Journal of Fisheries of China
基金
国家自然科学基金(U1605213)~~
关键词
鲍
荧光原位杂交
染色体
45S
rDNA
Haliotis
fluorescence in situ hybridization
chromosome
45S rDNA
