摘要
目的筛选肝细胞癌中程序性死亡蛋白1(PD-1)相关基因及生物学通路.方法从癌症基因组图谱(TCGA)下载369例肝细胞癌患者转库组测序(RNA-seq)数据,对PD-1相关基因进行基因本体(GO)生物进程分析、京都基因与基因组百科全书(KEGG)信号通路分析,并构建蛋白与蛋白相互作用网络图(PPI),获得PD-1的关键靶基因,并对这些靶基因进行生存分析.结果共筛选PD-1相关基因174个.功能富集分析表明这些基因共涉及239个生物学过程,12种细胞成分和12个分子功能,这些基因主要参与T细胞活化及调节、免疫缺陷、T细胞增殖等.同时筛选出10个肝细胞癌PD-1相关基因的靶基因,其中ZAP70和FASLG的低表达与肝细胞癌的不良预后显著相关(0.137±0.118比0.377±0.083,0.204±0.156比0.284±0.106,χ^2=6.900、4.400,P<0.05).结论PD-1相关基因为肝细胞癌免疫治疗提供新突破点.
Objective To screen the genes and biological pathways related to programmed death protein 1(PD-1)in hepatocellular carcinoma.Methods Download from the cancer genome atlas(TCGA)of 369 patients with hepatocellular carcinoma(HCC)transcriptome sequencing(RNA-seq)data,the PD-1 gene ontology(GO)to biological processes analysis,Kyoto encyclopedia(KEGG)signaling pathway gene and genome analysis,and construction of protein-protein interaction network diagram(PPI),key target genes for PD-1,and target genes for survival analysis.Results Altogether 174 PD-1 related genes were screened.Functional enrichment analysis showed that these genes involved 239 biological processes,12 cell components and 12 molecular functions.These genes were mainly involved in T cell activation and regulation,immune deficiency,T cell proliferation and so on.At the same time,10 key genes related to pd-1 in HCC were selected,and the low expression of ZAP70 and FASLG was significantly correlated with the poor prognosis of HCC(0.137±0.118 vs.0.377±0.083,0.204±0.156 vs.0.284±0.106,χ^2=6.900,4.400,P<0.05).Conclusion PD-1 gene provides a new breakthrough point for immunotherapy of hepatocellular carcinoma.
作者
任倩倩
朱鹏
龚昭
Ren Qianqian;Zhu Peng;Gong Zhao(Department of Radiology,Union Hospital,Tongji Medical Collage,Huazhong University of Science and Technology,Hubei Province Key Laboratory of Molecular Imaging,Wuhan 430022,China;Department of Hepatobiliary Surgery,Wuhan No.1 Hospital,Wuhan 430022,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2019年第12期2190-2192,共3页
Chinese Journal of Experimental Surgery
基金
湖北陈孝平科技发展基金(CXPJJH11800001-2018203)。
