摘要
基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)微生物识别分析系统现已发展为成熟的技术体系,其凭借简单、快速、经济、高通量以及高敏感性、高特异性的特点,在病原菌耐药性分析研究领域展现了巨大的潜力。近年来,基于MALDI-TOF MS的病原菌耐药性研究也取得了长足进展。本文将从定性和定量分析的角度阐述MALDI-TOF MS在病原菌耐药性分析领域的研究进展,并对其应用前景进行展望。
As a microorganism identification system,matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS)has been developed as a mature technique system.MALDI-TOF MS has shown great potential in the field of pathogen resistance analysis due to its simplicity,rapidity,cost-effectiveness,high throughput,high sensitivity and specificity.In addition to the wide use in bacterial identification,the researches on antibiotic resistance of pathogens based on MALDI-TOF MS have made great progress in recent years.This paper summarizes the progress in research of the application of MALDI-TOF MS in pathogen antibiotic resistance analysis from qualitative and quantitative perspectives and descripts the application prospect of this technique.
作者
杨文涛
张建中
肖迪
Yang Wentao;Zhang Jianzhong;Xiao Di(State Key Laboratory o f Communicable Disease Prevention and Control,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,National Institute for Communicable Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China;School of Public Health,Baotou Medical College,Baotou 014060,Inner Mongolia,China)
出处
《疾病监测》
CAS
2019年第11期974-979,共6页
Disease Surveillance
基金
国家科技重大专项（No.2018ZX10712001006012,No.2018ZX10733402003002）~~
关键词
基质辅助激光解吸电离飞行时间质谱
病原菌
耐药性分析
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Pathogen
Analysis of antibiotic resistance
