摘要
探讨T-2毒素对TM3细胞线粒体功能的影响。将不同浓度T-2毒素(0 nM、1 nM、10 nM、100 nM)作用于TM3细胞24 h,通过MTT法检测T-2毒素对TM3细胞增殖的影响;生化方法测定细胞内Ca^2+、活性氧、线粒体超氧化物、ATP的含量、Ca^2+-Mg^2+-ATPase和Na^+-K^+-ATPase的活性以及线粒体膜电位的变化。结果显示,T-2毒素对TM3细胞具有毒性作用,可引起细胞内Ca^2+含量增加,使细胞内活性氧和线粒体超氧化物的含量增加,降低线粒体膜电位、ATP的含量以及ATP依赖的Ca^2+-Mg^2+-ATPase和Na^+-K^+-ATPase的活性,进而影响线粒体功能的发挥。
To investigate the effects of T-2 toxin on mitochondrial function in TM3 cell.Different concentrations of T-2 toxin(0 nM,1 nM,10 nM,100 nM)were applied to TM3 cells for 24 h,and the effect of T-2 toxin on the proliferation of TM3 cells was detected by MTT assay.The contents of intracellular Ca^2+,reactive oxygen species.mitochondrial superoxide and ATP,the activities of Ca^2+-Mg^2+-ATPase and Na^+-K^+-ATPase and the changes of mitochondrial membrane potential were determined by biochemical methods.Results:T-2 toxin had toxic effects on TM3 cells,which could increase intracellular Ca^2+content,increase intracellular reactive oxygen and mitochondrial superoxide content,reduce mitochondrial membrane potential,ATP content and ATP-dependent Ca^2+-Mg^2+-ATPase and Na^+-K^+-ATPase,which in turn affected function of mitochondria.
作者
黄优
文银容
杨成林
徐泽河
郭江鹏
邬静
HUANG You;WEN Yinrong;YANG Chenglin;XU Zehe;GUO Jiangpeng;WU Jing(College of Veterinary Medicine,Hunan Agricultural University,Changsha Hunan 410128,China;Junshan Animal Health Supervision Institution,Yueyang Hunan 414000,China;Yueyang Animal Health Supervision Institution,Yueyang Hunan 414000,China)
出处
《中兽医医药杂志》
2019年第6期9-13,共5页
Journal of Traditional Chinese Veterinary Medicine
基金
国家自然科学基金(31572563)
湖南省大学生创新性实验计划项目(XCX18072)
湖南省教育厅科学研究项目(17B125)
