摘要
目的对比分析早发性卵巢功能不全(POI)小鼠模型三种常用造模方法的差异,探索符合POI临床特征的造模方法。方法将96只动情周期规律的ICR小鼠随机分为4组:A对照组、B雷公藤组、C二氧化乙烯基环己烯(VCD)组、D半乳糖组。适应性喂养后,分别给予不同造模干预,分别于干预后第16、46和76天随机处死各组8只小鼠,检测小鼠血清性激素、观察卵巢和子宫病理改变等。结果各模型组小鼠E2、卵泡数与对照组有显著差异;各模型组均出现动情周期紊乱,对照组则阴性。结论VCD腹腔注射法可损伤小鼠卵巢功能,造成小鼠POI模型;此造模方法简便易行、成功率高,在三种造模方法中成模更为理想,可推广。
Objective To evaluate the differences between three commonly used methods for establishing mouse models of premature ovarian insufficiency(POI),and to determine the method that is most accordant with the clinical features of POI.Methods Ninety-six ICR mice with regular estrous cycles were randomly divided into 4 groups:the control group(A),the tripterygium glycosides(TG)group(B),the 4-vinylcyclohexene diepoxide(VCD)group(C),and the D-(+)-galactose(D-gal)group(D).After intervention with the different chemicals,eight mice in each group were sacrificed,the serum sex hormone levels were measured,and the pathological changes of uterus and ovaries were examined.Results The level of E2 and the number of follicles differed significantly between mice in the model groups and those in the control group(P<0.01).And the estrous cycles in mice of the model groups were significantly irregular whereas those of the control group were regular.Conclusions Mouse models of POI can be successfully established with VCD via intraperitoneal injection.This method has the advantages of being an easier operation and having a higher success rate,as compared with the other two models method.It is the ideal one of the three establishing method and is suitable for popular use.
作者
苏先芝
刘一斐
孔文娟
闫菲
王晓梅
韩福国
刘清飞
史云
SU Xianzhi;LIU Yifei;KONG Wenjuan;YAN Fei;WANG Xiaomei;HAN Fuguo;LIU Qingfei;SHI Yun(the First Clinical Medical College,Beijing University of Chinese Medicine,Beijing 100029,China;School of Pharmaceutical Sciences,Tsinghua University,Beijing 100084;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2019年第6期725-732,共8页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金(81202717)
科技部国际合作重点项目(2016YFE0113700)~~
关键词
早发性卵巢功能不全
二氧化乙烯基环己烯
造模方法
雷公藤
半乳糖
小鼠
premature ovarian insufficiency(POI)
vinylcyclohexene dioxide(VCD)
mouse models
establishing method
tripterygium glycosides(TG)
D-(+)-galactose(D-gal)
