摘要
植物酶诱导农药在植物中的代谢转化对农药的安全使用及风险评估具有重要意义。现有研究表明,农药在植物中的代谢转化途径主要分为3个阶段:I相代谢为氧化、水解和还原等酶的催化及非生物降解过程;II相代谢是在谷胱甘肽S-转移酶、糖基转移酶和丙二酰基转移酶等作用下农药及其代谢物与植物内源物质进行的轭合过程;III相代谢中,植物细胞质中的轭合物被分泌到液泡溶质储存,或运输到质外体中作为结合残留物保留。文章综述了植物中的农药在植物酶作用下的3相代谢转化研究进展,包括参与代谢的植物酶系、基因鉴定、代谢途径及调控机制、研究方法及相关应用等,旨在为解析农药在植物中的归趋、农药膳食风险评估、农药抗性管理及环境修复等相关研究提供理论及技术参考。
The metabolic transformations of pesticides in plants induced by plant enzymes are crucial for their application safety and risk assessment.Existing studies demosntrated that the metabolic pathways of pesticides in plants were mainly divided into three stages.Phase I reactions include oxidation,hydrolysis,reduction and other processes,which were either catalysed by enzymes or were abiotic degradation processes.Phase II reactions were conjugation reactions between pesticides and/or their metabolites with endogenous substances in the plant via the action of glutathione S-transferases,glycosyltransferases,malonyltransferases,and etc.In Phase III metabolism,the conjugates were secreted from cytosol into the vacuole or apoplast in plant,which were stored as a solutes or bound residues.In this review,the recent progress in the three-phase metabolic transformations of pesticides in plants,which is mediated by plant enzymes,has been summaried.And also covers plant enzymes involed in the metabolic processes,gene identification,metabolic pathways and regulatory mechanisms,and both the research methods and research applications were reviewed.This paper will provide theoretical and technical references for relevant research for analyzing the fate of pesticides in plant,dietary risk assessment of pesticides,resistance management of pesticide and environmental remediation.
作者
李如男
董丰收
吴小虎
刘新刚
徐军
郑永权
LI Runan;DONG Fengshou;WU Xiaohu;LIU Xingang;XU Jun;ZHENG Yongquan(Institute of Plant Protection,Chinese Academy of Agricultural Sciences,State Key Laboratory for Biology of Plant Diseases and Insect Pests,Beijing 100193,China)
出处
《农药学学报》
CAS
CSCD
北大核心
2019年第5期799-814,共16页
Chinese Journal of Pesticide Science
基金
国家重点研发计划(2016YFD0200204)
关键词
农药代谢
植物酶
细胞色素P450酶
谷胱甘肽S-转移酶
糖基转移酶
丙二酰基转移酶
环境修复
metabolism of pesticide
plant enzymes
cytochrome P450 enzyme
glutathione Stransferases
glycosyltransferases
malonyltransferases
environmental remediation
