摘要
采用异丙肾上腺素诱导心肌缺血大鼠模型,使用液相色谱-质谱法检测血清和心肌中的内源性成分,应用软件对已鉴定的40余种目标成分进行靶向提取,用主成分分析(PCA)、有监督偏最小二乘法判别分析(PLS-DA)对代谢组学数据进行多维度统计分析,筛选潜在生物标志物。与对照组相比,在心肌缺血模型组大鼠血清、组织中检测出18个差异代谢物,涉及精氨酸和脯氨酸代谢、甘氨酸、丝氨酸和苏氨酸代谢、谷氨酰胺和谷氨酸代谢、牛磺酸和亚牛磺酸代谢等多条代谢通路。代谢产物可作为心肌缺血研究中的重要标志物,该研究结果有助于揭示心肌缺血的发病机制,可为临床疾病诊断提供思路。
A rat model with isoproterenol(ISO)-induced myocardial ischemia was obtained. Liquid chromatography-mass spectrometry(LC-MS)was conducted on the serum and heart tissue metabolites of normal and model rats. Principal component analysis(PCA) and differentiation analysis of supervised partial least-squares method(PLS-DA) were applied to the metabolomics data for multidimensional statistical analysis, and the resulting biomarkers were screened. Compared with the normal group, 18 different endogenous metabolites in plasma and tissues were detected, including the products of arginine and proline metabolism;glycine, serine, and threonine metabolism;glutamine and glutamate metabolism;and taurine and hypotaurine metabolism. These metabolites can be used as important biomarkers for myocardial ischemia. The results of this study can help reveal the pathogenesis of myocardial ischemia and aid clinical diagnosis.
作者
尹春园
孙明谦
金龙
林力
苗兰
刘建勋
YIN Chunyuan;SUN Mingqian;JIN Long;LIN Li;MIAO Lan;LIU Jianxun(Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China;Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《色谱》
CAS
CSCD
北大核心
2020年第2期206-211,共6页
Chinese Journal of Chromatography
基金
国家重点基础研究发展计划(“973”计划)(2015CB554405)
国家自然科学基金(81872992,81673587,81803770)~~
关键词
液相色谱-质谱
生物标志物
代谢组学
心肌缺血
liquid chromatography-mass spectrometry(LC-MS)
biomarkers
metabolomics
myocardial ischemia
