摘要
目的建立一种从甘草超滤液中制备甘草素的工艺路线。方法以甘草素保留率为指标,采用U5(53)均匀设计优化超滤工艺;以萃取率为指标,采用Box-Behnken响应面法优化络合萃取工艺;以甘草素反萃取率为指标,确定反萃取甘草素的工艺条件。结果甘草素的最佳超滤条件为孔径10 nm的无机陶瓷膜、压力0.12 MPa、料液温度25℃,甘草素保留率98.74%;1%三烷基氧化膦(TRPO)、萃取10 min、5 mL有机相溶液为最佳络合萃取条件,甘草素萃取率99.44%;12.5 mmol/L NaOH水溶液为最佳反萃取剂,反萃取率98.88%。结论作为一种新兴的中药萃取技术,超滤-络合萃取反萃取技术具有高选择、高效、萃取剂循环利用等优点,可为甘草素的研究提供一种全新的制备技术。
Objective A technological route was established for preparing liquiritigenin from the licorice ultrafiltrate.Methods U5(53)uniform design was used to optimize the ultrafiltration process with the retention rate of liquiritigenin as the index;Box-Behnken response surface method was used to optimize the complexation extraction process with the extraction rate of liquiritigenin as the index;The technological condition of back extraction of liquiritigenin was determined by the liquiritigenin stripping rate.Results The optimal ultrafiltration conditions for liquiritigenin were inorganic ceramic membranes with a pore size of 10 nm,a pressure of 0.12 MPa,and solution temperature of 25℃,retention rate of liquiritigenin was 98.74%.Optimum complexation extraction conditions were as following:1%TRPO complexing agent,extraction for 10 min in 5 mL organic phase solution,the extraction rate of liquiritigenin was 99.44%;The 12.5 mmol/L NaOH aqueous solution was the best back-extractant,and the back extraction rate was 98.88%.Conclusion As a new Chinese medicine extraction technology,ultrafiltration-complexation extraction and back extraction technology have the advantages of high selectivity,high efficiency,and recycling of extractant,which can provide a new preparation technology for the research of liquiritigenin.
作者
石盘棋
刘晓霞
孙晓燕
王继龙
魏舒畅
周博
蒲红利
SHI Pan-qi;LIU Xiao-xia;SUN Xiao-yan;WANG Ji-long;WEI Shu-chang;ZHOU Bo;PU Hong-li(Research Center of traditional Chinese Medicine Pharmaceutical Technology and Engineering of Gansu Province,Gansu University of Chinese Medicine,Lanzhou 730000,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第23期5739-5744,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目（81460608）
甘肃省基础研究创新群体项目（1506RJIA034）
关键词
甘草素
超滤
络合萃取
反萃取
三烷基氧化膦
磺化煤油
响应面法
liquiritigenin
ultrafiltration
complexation extraction
back extraction
trialkylphosphine oxide
sulfonated kerosene
response surface method
