摘要
目的:观察中药贴敷双侧"天枢"穴对功能性便秘大鼠结肠肌间神经丛血管活性肠肽(VIP)、P物质(SP)的影响,探讨中药穴位贴敷改善功能性便秘的机制。方法:SD大鼠随机分正常组、模型组、穴位贴敷组、药物组,每组10只。采用盐酸洛哌丁胺混悬液(0.5 mg/mL)灌胃法复制功能性便秘大鼠模型。穴位贴敷组予双侧"天枢"穴中药贴敷治疗,6 h/d,每日1次,药物组每日予莫沙必利混悬液(0.15 mg/mL)灌胃,均治疗4周。记录各组大鼠首粒黑便排出时间、6 h内大便粒数和含水量。免疫组织化学法检测大鼠结肠肌间神经丛VIP、SP表达。结果:与正常组相比,模型组大鼠首粒黑便排出时间延长,6 h内大便粒数及含水量显著减少(P<0.01),结肠肌间神经丛VIP、SP阳性表达减少(P<0.01)。与模型组比较,穴位贴敷组与药物组大鼠首粒黑便排出时间缩短,6 h内大便粒数和含水量增加(P<0.01),结肠肌间神经丛VIP、SP阳性表达升高(P<0.01)。穴位贴敷组与药物组比较差异无统计学意义(P>0.05)。结论:中药穴位贴敷可通过调节肠神经递质,恢复结肠动力,从而有效改善便秘大鼠的排便功能。
Objective To observe the effect of acupoint application at bilateral"Tianshu"(ST25)on intestinal mobility and immunoactivity of vasoactive intestinal peptide(VIP)and substance P(SP)in colonic myenteric plexus of rats with functional constipation(FC),so as to analyze its mechanisms underlying improving FC.MethodsForty male SD rats were randomly divided into 4 groups,namely normal control,model,acupoint application and medication,with 10 rats in each group.The FC model was established by gavage of Loperamide Hydrochloride suspension fluid(0.5 mg/mL,3 mg·kg-1·d-1)for 7 days.Herbal medicine paste(composed of Rheum Officinale,Sodium Sulfate,Mangnolia Officinalis,etc.)was applied to bilateral ST25 for 6 h,once daily for 4 weeks.Rats of the medication group were treated by gavage of Mosapride suspension fluid(0.15 mg/mL,1.58 mg·kg-1·d-1)for 4 weeks.After the treatment,the rats were deprived of water for 12 hours,and then treated by gavage of 2 mL of activated carbon suspension,followed by recording the first black defecation time and the number of fecal particles and water content of feces within 6 h so as to assess the intestinal mobility.The immunoactivity and average surface density of VIP and SP positive granules in the colonic myenteric plexus were detected by immunohistochemistry.ResultsCompared with the normal control group,the first black defecation time was significantly prolonged,and the number and water content of fecal particles within 6 h,and the expression and the average surface density of VIP and SP were significantly reduced in the model group(P<0.01).After the treatment and compared with the model group,the first black defecation time was shortened,and the fecal water content and fecal particle number within 6 h,as well as the expression and the average surface density of VIP and SP were considerably increased in both acupoint application and medication groups(P<0.01).There were no significant differences between the acupoint application and medication groups in all the indexes mentioned above after the interventions(P>0.05).ConclusionAcupoint application may improve the intestinal motility in FC rats,which may be associated with its effects in up-regulating the immunoactivity of VIP and SP in colonic myenteric plexus of the large intestine.
作者
孟肖蒙
刘晓亭
MENG Xiao⁃meng;LIU Xiao⁃ting(Acupuncture⁃moxibustion and Tuina College,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)
出处
《针刺研究》
CAS
CSCD
北大核心
2019年第12期906-910,共5页
Acupuncture Research
基金
辽宁省教育厅科学研究项目(No.L201703)
关键词
功能性便秘
天枢
中药穴位贴敷
血管活性肠肽
P物质
Functional Constipation
Tianshu(ST25)
Acupoint application with Chinese herbal medicine
Va-soactive intestinal peptide
Substance P
