摘要
转录组测序技术是一种新发展的转录组学研究方法。在物种基因组信息未知的情况下,进行测序得到遗传信息,转录组测序技术是很重要的分子生物学方法,已经被广泛应用于各个研究领域。而药用植物遗传信息匮乏,对药用植物的转录组进行测序在基因组学是比较活跃的领域,可以了解植物的基因表达并分析其功能和调控机制。对药用植物的转录组学研究有助于解决遗传育种、筛选优良抗性基因等问题。介绍了转录组测序的发展,并从功能基因挖掘和次生代谢产物途径探索等方面综述了近年来转录组测序在药用植物研究中的应用,为药用植物的研究提供了更多基础数据。
Transcriptome sequencing technology is a newly developed transcriptomics method.In the absence of the genome information of the species,sequencing is carried out to obtain genetic information.Transcriptome sequencing technology is an important molecular biology method with wide application in various research fields.Due to the deficience of the genetic information of medicinal plants,and the transcriptome sequencing research of medicinal plants is an active field in genomics.Researchers can investigate plant gene expression and analyze its function and regulatory mechanisms.Transcriptomics research on medicinal plants can help to solve problems such as genetic breeding,excellent resistance genes screening,and etc.This paper introduces the development of transcriptome sequencing,and reviews the application of transcriptome sequencing in medicinal plants in recent years from functional gene mining and secondary metabolite pathway exploration,which provide more basic data for medicinal plant studies.
作者
慧芳
刘秀岩
李宗谕
刘福顺
杨世海
HUI Fang;LIU Xiu-yan;LI Zong-yu;LIU Fu-shun;YANG Shi-hai(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第24期6149-6155,共7页
Chinese Traditional and Herbal Drugs
基金
国家中医药管理局中药标准化项目：甘草等7种中药饮片标准化建设(ZYBZH-Y-JL-25)
关键词
药用植物
转录组测序
功能基因
次生代谢产物
遗传育种
筛选优良抗性基因
medicinal plant
transcriptome sequencing technology
functional genes
secondary metabolite
genetic breeding
screening for excellent resistance genes
