摘要
目的Allergy Lateral Flow Assay(ALFA)是一种便捷、小巧的过敏原检测新技术,本研究以荧光酶联免疫全定量ImmunoCAP法为“金标准”,旨在探讨ALFA的检验效能及在中国南方地区的应用价值。方法利用ALFA技术与ImmunoCAP技术检测100例过敏性疾病患者血清屋尘螨、粉尘螨、热带无爪螨、猫毛皮屑、狗毛皮屑、蟑螂和艾蒿sIgE水平。结果以ImmunoCAP法为“金标准”,结果显示,ALFA检测粉尘螨的一致性最高(κ=0.56,P<0.05),其次是屋尘螨(κ=0.43,P<0.05);同时,ALFA检测粉尘螨(87.6%)、猫毛皮屑(73.7%)和屋尘螨(87.6%)的总一致率均较高。经受试者工作特征曲线分析显示,ALFA诊断螨类过敏原的曲线下面积均大于0.90(P<0.05)。对于屋尘螨、粉尘螨和猫毛皮屑过敏原,ALFA与ImmunoCAP系统的sIgE检测结果具有强相关性(r I>0.8,P<0.05)。结论ALFA具有良好的诊断效能,可满足临床检测要求,因其无须昂贵的检测仪器和检测费用,可为基层医院临床筛查应用提供新的选择。
Objective Allergy Lateral Flow Assay(ALFA)is a convenient and compact new allergen detection technology.This study uses the fluorescent enzyme-linked immunoquantitative ImmunoCAP method as the gold standard to explore the efficacy of ALFA and its application value in southern China.Methods ALFA technique and ImmunoCAP technique were used to detect the levels of sIgE in serum dust mites,dust mites,tropical gnaws,cat fur,dog fur,cockroaches and mugwort in 100 patients with allergic diseases.Results Based on the ImmunoCAP system,the results showed that ALFA detected the highest consistency of dust mites(κ=0.56,P<0.05),followed by house dust mites(κ=0.43,P<0.05).At the same time,ALFA detected dust mites(87.6%),cat fur shavings(73.7%)and house dust mites(87.6%)had higher overall compliance rates.ROC curve analysis showed that the area under the curve of ALFA diagnosis of mites allergens was greater than 0.90(P<0.05).For household dust mites,dust mites and cat litter allergens,ALFA had a strong correlation with the sIgE test results of the ImmunoCAP system(r I>0.8,P<0.05).Conclusion ALFA has good diagnostic performance and can meet the requirements of clinical testing.It can provide new choices for clinical screening applications in primary hospitals because it does not require expensive testing instruments and testing costs.
作者
陈辉雄
胡海圣
罗文婷
吴泽鸿
孙宝清
CHEN Huixiong;HU Haisheng;LUO Wenting;WU Zehong;SUN Baoqing(Guangzhou Medical University,Guangzhou,Guangdong 510120,China;Longgang District People′s Hospital,Shenzhen,Guangdong 518172,China;State Key Laboratory of Respiratory Disease/Department of Allergy,the First Affiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong 510120,China)
出处
《国际检验医学杂志》
CAS
2020年第2期141-145,共5页
International Journal of Laboratory Medicine
基金
广医大高水平大学建设科研教学学术提升项目(B185004118)
