摘要
目的探讨黄芪总苷防治氯胺酮麻醉致幼鼠记忆损伤的作用及机制。方法SD幼年大鼠(幼鼠)50只,随机分为正常对照组、氯胺酮组、氯胺酮+黄芪总苷高剂量(80mg/kg)组,氯胺酮+黄芪总苷中剂量(40mg/kg)组、氯胺酮+黄芪总苷低剂量(20mg/kg)组,每组10只。采用腹腔注射氯胺酮(50mg/kg)麻醉并行剖腹探查术,制造记忆损伤模型;造模后,氯胺酮+黄芪总苷各剂量组分别灌胃给药相应剂量黄芪总苷,正常对照组和氯胺酮组灌胃等体积的0.9%氯化钠溶液;末次给药后,进行定向航行实验和空间探索实验,并检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)水平,同时取大鼠脑组织,分别用于HE染色观察病理变化和Western blot法检测caspase-3、Bcl-2蛋白表达水平。结果成功复制幼鼠记忆损伤模型;与正常对照组比较,氯胺酮组幼鼠血清SOD活性显著下降,MDA含量显著提升(P<0.05);与氯胺酮组比较,氯胺酮+黄芪总苷各剂量组幼鼠血清SOD活性显著提升,MDA含量显著降低(P<0.05);病理组织学检查结果显示,氯胺酮+黄芪总苷各剂量组幼鼠大脑组织海马区神经元细胞形态明显改善;与氯胺酮组比较,氯胺酮+黄芪总苷各剂量组幼鼠脑组织caspase-3蛋白表达水平均显著降低(P<0.05),而Bcl-2表达水平均显著升高(P<0.05)。结论黄芪总苷对氯胺酮麻醉致幼鼠记忆损伤具有显著的防治作用,其机制可能与调节脑组织海马区caspase-3、Bcl-2等凋亡蛋白表达、抑制氧化应激反应有关。
Objective To investigate the effect and mechanism of astragaloside on memory impairment induced by ketamine anesthesia in young rats.Methods Fifty SD rats were randomly divided into normal control group,ketamine group,ketamine+astragaloside high dose group(80mg/kg),ketamine+astragaloside medium dose group(40mg/kg),ketamine+astragaloside low dose group(20mg/kg),10 rats in each group.The memory impairment model was made by intraperitoneal injection of ketamine(50mg/kg)anesthesia and laparotomy.After the establishment of the model,the corresponding dose of astragaloside was treated by intragastric administration in each dose group of ketamine+tastragaloside.The rats in the normal control group and the ketamine group were treated with the same volume of saline.After the last administration,the effect and mechanism of Astragaloside on memory impairment was explored by the directional navigation and space exploration experiments.The levels of SOD and MDA in serum were detected.At the same time,rat brain tissues were taken for HE staining to observe pathological changes.The expression levels of caspase-3 and Bcl-2 protein were detected by Western blotting.Results The model of memory impairment in the rats was successfully replicated.Compared with the normal control group,the activity of SOD and the content of MDA in serum of the rats in the ketamine group decreased significantly(P<0.05).Compared with the ketamine group,the activity of SOD and the content of MDA in serum of the rats in the ketamine+astragaloside groups increased significantly(P<0.05).The results of histopathological examination showed that the morphology of hippocampal neurons was significantly improved in all dose groups of ketamine+astragaloside,and the expression of caspase-3 protein was significantly decreased(P<0.05),while the expression of Bcl-2 was significantly increased(P<0.05).Conclusion Astragaloside has a significant preventive and therapeutic effect on memory impairment induced by ketamine anesthesia in young rats.Its mechanism may be related to regulating the expression of caspase-3 and Bcl-2 apoptotic proteins in brain tissue and inhibiting oxidative stress response.
作者
杨飞
钮峥嵘
李瑞轩
徐桂萍
Yang Fei;Niu Zhengrong;Li Ruixuan(Department of Anesthesiology,The Xinjiang Uygur Autonomous Region People′s Hospital,Xinjiang 830001,China)
出处
《医学研究杂志》
2019年第12期65-68,72,共5页
Journal of Medical Research
基金
新疆维吾尔自治区自然科学基金资助项目(2017D01C147)
关键词
黄芪总苷
氯胺酮
幼鼠
记忆损伤
氧化应激
细胞凋亡
Astragaloside
Ketamine
Young rats
Memory impairment
Oxidative stress
Apoptosis
