摘要
通过对秘鲁茎柔鱼及澳洲双柔鱼的线粒体16S rRNA基因片段进行聚合酶链式反应(polymerase chain reaction,PCR)扩增和序列比对,确定2种柔鱼品种的特异性位点,并由此设计品种特异性引物。利用多重PCR体系对秘鲁茎柔鱼和澳洲双柔鱼进行快速、准确地鉴定和区分,并对该体系的灵敏度进行检验。结果表明:在多重PCR体系下,秘鲁茎柔鱼和澳洲双柔鱼分别扩增出长度400、229 bp的品种特异性条带,2种柔鱼的混合样品则同时扩增出长度400、229 bp的条带;且该多重PCR体系可以检测澳洲双柔鱼中1%秘鲁茎柔鱼的掺入,检测限为0.1 ng。
The aim of this study is to establish a rapid and accurate method for molecular discrimination of two squid species, Nototodarus gouldi and Dosidicus gigas. The fragments of mitochondrial 16S rRNA were amplified by polymerase chain reaction(PCR) from the genomic DNA of each squid species. Polymorphic sites were determined by multiple sequence alignments and species-specific primers were designed. Multiplex PCR was conducted for molecular identification of the two squid species with the species-specific primer pairs. Multiplex PCR results showed that specific amplicons of 400 and 229 bp were generated from D. gigas and N. gouldi, respectively. Both 400 and 229 bp amplicons were obtained from the mixtures of the two species. The sensitivity test results showed that the method could detect D. gigas adulteration at 1%in N. gouldi with a limit of detection of 0.1 ng.
作者
田潇然
吕世鑫
陈玉春
王洪涛
TIAN Xiaoran;LÜShixin;CHEN Yuchun;WANG Hongtao(School of Life Sciences,Yantai University,Yantai 264005,China)
出处
《肉类研究》
北大核心
2020年第1期64-68,共5页
Meat Research
基金
烟台大学研究生科技创新基金项目
