摘要
考察金线莲多糖抗衰老作用及其机制.每天给小鼠皮下注射D-半乳糖溶液(40 mg·kg-1)诱导其衰老,同时灌胃给予金线莲多糖(100,200,300 mg·kg-1)或维生素E(200 mg·kg-1)进行治疗,以维生素E治疗组为阳性对照.6周后对小鼠进行行为学测试,8周后检测小鼠的大脑皮层p-NF-κB p65表达,以及大脑皮层过氧化氢酶、超氧化物歧化酶及血清总抗氧化能力活性和丙二醛浓度,并检测小鼠腹腔巨噬细胞的吞噬情况.结果表明:与衰老小鼠相比较,经金线莲多糖治疗后,小鼠的抗氧化酶活性显著提高,其大脑皮层p-NF-κB p65表达下调,且运动、空间探索和学习记忆能力明显改善;同时,金线莲多糖能增强衰老小鼠清除抗原的能力;金线莲多糖抗衰老作用与其抗氧化、抑制NF-κB信号通路、增强衰老小鼠免疫能力有关.
The anti-aging effect and mechanisms of Anoectochilus roxburghii polysaccharose(ARP)was studied.The aging mouse model was induced by subcutaneous injection of D-galactose solution(40 mg·kg-1)daily,then the treated mice were intragastrically administered with ARP(100,200 and 300 mg·kg-1)or Vitamin E(200 mg·kg-1),and Vitamin E treatment group was used as a positive control.After 6 weeks,the behavioral tests were performed.All mice were sacrificed after 8 weeks,and the expression of p-NF-κB p65 and antioxidant enzymes including superoxide dismutase and catalase in the cerebral cortex were examined.The activity of total antioxidant enzyme activity and malondialdehyde content in serum were measured and the phagocytic ability of mouse peritoneal macrophages were detected.The results showed that compared with aging mice,treatment with ARP could increase the activity of antioxidant enzymes significantly and reduce p-NF-κB p65 expression in cerebral cortex,and increase the movement abilities,space exploration,learning and memory abilities of aging mice.Meanwhile,ARP could enhance the ability of aging mice to clear antigen.The ARP anti-aging effect was due to its antioxidant effect,the inhibition of NF-κB signaling pathway and the improved immunity.
作者
刘青
李永
盛世美
LIU Qing;LI Yong;SHENG Shimei(College of Chemical Engineering,Huaqiao University,Xiamen 361021,China)
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2020年第1期77-83,共7页
Journal of Huaqiao University(Natural Science)
基金
福建省自然科学基金资助项目(2013J01337)。
关键词
金线莲多糖
NF-ΚB信号通路
抗氧化
认知能力
抗衰老
Anoectochilus roxburghii polysaccharose
NF-κB signaling pathway
antioxidant
cognitive ability
anti-aging
