摘要
目的观察山楂叶总黄酮对心梗后慢性心力衰竭(由瘀致虚证)模型大鼠左室射血分数(left ventricular ejection fraction,LVEF)和血浆AngⅡ、ALD水平的影响。方法选用雄性SD大鼠,结扎冠状动脉前降支术造成心肌梗死,假手术组穿线但不结扎冠状动脉。造模成功4周后存活的大鼠80只随机等分成8组:模型组、西药对照组、中药干预组亚组1~3、中西结合组亚组1~3。假手术组和模型组给予生理盐水;西药对照组给予卡托普利10 mg/(kg·d);中药干预组:亚组给予山楂叶总黄酮低剂量50 mg/(kg·d);亚组2给予山楂叶总黄酮中剂量100 mg/(kg·d);亚组3给予山楂叶总黄酮高剂量200 mg/(kg·d);中西结合组:亚组1给予山楂叶总黄酮低剂量50 mg/(kg·d)+卡托普利10 mg/(kg·d);亚组2给予山楂叶总黄酮中剂量100 mg/(kg·d)+卡托普利10 mg/(kg·d);亚组3给予山楂叶总黄酮高剂量200 mg/(kg·d)+卡托普利10 mg/(kg·d),以上各组均制成同等容量(3 mL)灌胃液,连续喂养至第8周。8周后全部大鼠测定LVEF值的变化和血浆AngⅡ、ALD水平。结果与假手术组比较,模型组的LVEF值显著降低(P<0.01),血浆AngⅡ、ALD水平均显著增高(P<0.01);与模型组比较,中药干预组亚组1、亚组2 LVEF值和血浆AngⅡ、ALD水平差异无统计学意义(P>0.05),中西结合组各亚组、中药干预组亚组3、西药对照组LVEF值和血浆AngⅡ、ALD水平均有显著差异(P<0.05);与西药对照组比较,中药干预组亚组3的LVEF值和血浆AngⅡ、ALD水平差异无统计学意义(P>0.05),中西结合组亚组3的血浆AngⅡ、ALD水平有显著差异(P<0.01)。结论山楂叶总黄酮高剂量与卡托普利联用的中西医结合治疗能提高心梗后慢性心力衰竭(由瘀致虚证)模型大鼠左室射血分数,降低其血浆AngⅡ、ALD水平,疗效明显优于单独使用卡托普利或单独使用高剂量山楂叶总黄酮。
Objective To observe the effects of Hawthorn leaf flavonoids on left ventricular ejection fraction(LVEF) and plasma Ang Ⅱ and ALD levels in rats with chronic heart failure after myocardial infarction(due to stasis-induced deficiency syndrome). Methods Myocardial infarction was induced by ligation of anterior descending coronary artery in male SD rats. Eighty rats survived 4 weeks after successful modeling were randomly divided into 8 groups: model group, western medicine control group, Chinese medicine intervention group. Sham operation group and model group were given normal saline, western medicine control group was given captopril 10 mg/(kg ·d);intervention group: subgroup was given low dose 50 mg/(kg ·d) of Hawthorn leaf total flavonoids. Subgroup 2 was given 100 mg/(kg ·d);subgroup 3 high dose 200 mg/(kg ·d);combination group: subgroup 1 was given to Hawthorn leaves with low dose 50 mg/(kg ·d) captopril 10 mg/(kg ·d). Subgroup 2 was treated with 100 mg/(kg ·d) captopril 10 mg/(kg ·d);at the middle dose of total flavonoids from Hawthorn leaves. Subgroup 3 was fed with high dose of 200 mg/(kg ·d) captopril 10 mg/(kg ·d), into gastric juice of equal volume(3 mL) for 8 weeks. After 8 weeks, all rats were determined LVEF value and plasma Ang Ⅱ, ALD levels. Results Compared with the sham-operated group, the LVEF value in the model group decreased significantly(P<0.01), and the levels of Ang Ⅱ and ALD in the plasma increased significantly(P<0. 01). Compared with the model group, there was no significant difference in the levels of Ang Ⅱ and ALD between the two groups(P>0.05). There was no significant difference in the levels of plasma Ang Ⅱ and ALD between the Chinese medicine intervention group and the western medicine intervention group(P>0.05). There was no significant difference in the levels of Ang Ⅱ and ALD in the Chinese medicine intervention group, the Chinese medicine intervention group and the western medicine control group. There was a significant difference in the level of ALD between the two groups(P<0.05). Compared with the western medicine control group, the LVEF value and plasma Ang in subgroup 3 of the Chinese medicine intervention group were significantly higher than those in the control group. There was no significant difference in the levels of Ang Ⅱ and ALD between the two groups(P>0.05). There was a significant difference in plasma Ang Ⅱ and ALD levels between the two groups(P<0.01). Conclusion High dose of total flavonoids from Hawthorn leaves combined with captopril can increase left ventricular ejection fraction and decrease plasma Ang Ⅱ and ALD levels in rats with chronic heart failure after myocardial infarction(due to stasis-induced deficiency syndrome). The curative effect is superior to captopril alone or high-dose Hawthorn leaf total flavonoids alone.
作者
贺粤
周亚滨
景慎鸿
HE Yue;ZHOU Ya-bin;JING Shen-hong(Graduate School,Heilongjiang University of Traditional Chinese Medicine;Department of Cardiovascular,The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;Department of Cardiology,The Second Affiliated Hospital of Harbin Medical University,Harbin 150081,China)
出处
《哈尔滨医科大学学报》
CAS
2019年第5期469-473,共5页
Journal of Harbin Medical University
基金
黑龙江省中医药科研项目(3-54)
关键词
山楂叶总黄酮
卡托普利
慢性心力衰竭
心功能
心室重构
肾素-血管紧张素-醛固酮系统
Hawthorn leaf total flavonoids
captopril
chronic heart failure
cardiac function
ventricular remodeling
renin-angiotensin-aldosterone system
