摘要
无菌播种繁殖是兰花新品种选育的关键技术。为有效实现兰花杂交子代的无菌播种繁殖,以墨兰新品种“梦之兰”杂交种子为试材,对其原球茎诱导培养基、增殖培养基、分化培养基和生根壮苗培养基进行筛选优化。结果表明,原球茎诱导最佳培养基为改良MS+6-BA 3.0 mg·L^-1+NAA 1.5 mg·L^-1,原球茎增殖培养最佳培养基为改良MS+6-BA 2.0 mg·L^-1+KT 2.5 mg·L^-1+NAA 1.5 mg·L^-1,原球茎分化最佳培养基为改良MS+6-BA 3.0 mg·L^-1+KT 3.0 mg·L^-1+NAA 1.5 mg·L^-1,生根壮苗培养最佳培养基为改良1/2MS+NAA 1.0 mg·L^-1+IBA 1.5 mg·L^-1+AC 2.0 g·L^-1。
Sterile sowing and breeding is the key technology for breeding new orchid varieties.In order to effectively realize the aseptic sowing and propagation of orchid hybrid progeny,this study used the hybrid seed of Mengzhilan as the test material,and its original bulb induction medium,proliferation medium and differentiation medium,rooting and strong seedling medium were optimized to screening.The results showed that the optimal medium of protocorm induction was modified MS+6-BA 3.0 mg·L^-1+NAA 1.5 mg·L^-1,and the best medium of protocorm proliferation was modified MS+6-BA 2.0 mg·L^-1+KT 2.5 mg·L^-1+NAA 1.5 mg·L^-1,the best medium of protocorm differentiation was improved MS+6-BA 3.0 mg·L^-1+KT 3.0 mg·L^-1+NAA 1.5 mg·L^-1,the best medium of rooting and strong seedling was modified 1/2MS+NAA 1.0 mg·L^-1+IBA 1.5 mg·L^-1+AC 2.0 g·L^-1.
作者
张月娇
ZHANG Yuejiao(Fujian Forestry Science and Technology Test Center,Nanjing,Fujian 363600)
出处
《武夷学院学报》
2019年第12期9-13,共5页
Journal of Wuyi University
基金
林业科技(示范)园区项目(闽林科便函[2019]16号)
