摘要
目的:分析草乌与生半夏、法半夏配伍反与不反及其化学成分。方法:Bliss法对法半夏和草乌合煎液、生半夏和草乌合煎液及草乌提取液的LD50进行计算;采用UPLC-Q-TOF-MS对两合煎液成分进行分析,经OPLS-DA和PCA对两合煎液内中毒性成分间差异行统计分析。结果:法半夏和草乌提取液对于小鼠Dm是16.96g/kg,Dn是8.50g/kg;生半夏和草乌提取液对于小鼠Dm是13.97g/kg,Dn是1.85g/kg;草乌提取液对于小鼠Dm是14.98g/kg,Dn是2.30g/kg;法半夏和草乌合煎液LD50为12.20g/kg,其置信区间是11.36~13.10g/kg;生半夏和草乌合煎液LD50为4.39g/kg,其置信区间是3.69~5.28g/kg;草乌煎液LD50为7.97g/kg,其置信区间是6.97至9.09g/kg。和单独使用草乌相比,法半夏配伍草乌的LD50变大,生半夏配伍草乌的LD50变小;2组间有13个化学成分存在差异,生半夏和草乌合煎液内,峰13(去氧乌头碱)、峰9(北草乌碱)、峰12(10-脱乙酰乌头碱)、峰11(次乌头碱)和峰10(新乌头碱)是双酯型生物碱;峰8(苯甲酰次乌头原碱)、峰6(苯甲酰新乌头原碱)、峰4(10-OH-苯甲酰新乌头原碱)是单酯型生物碱显著增大;峰3(阿克塔林)及峰1(醇胺型生物碱中乌头胺)增加不显著,峰2(尼奥林)显著降低,另2个未知成分增加不显著。结论:相比单用草乌,法半夏和草乌配伍后其毒性下降,生半夏和草乌配伍后其毒性升高;草乌和法半夏或者生半夏配伍后其毒性升高或者降低与酯型生物碱含量有联系。
Objective:To analyze the anti-and non-reverse chemical constituents of Radix Aconiticum Maxim. Methods:Bliss method was used to calculate the LD50 of the method of Pinellia ternata and Caowu Decoction, raw Pinellia ternata and Caowu Decoction and Caowu extract. UPLC-Q-TOF-MS was used to analyze the composition of the two combined decoction. The statistical analysis of the differences between the toxic components in the two decoctions by OPLS-DA and PCA. Results:The extracts of Pinellia ternata and Radix Scutellariae were 16.96 g/kg for Dm and 8.50 g/kg for Dn. The extracts of Pinellia ternata and Radix Salviae Miltiorrhizae were 13.97 g/kg for Dm and 1.85 g/kg for Dn. kg;grass extracts for mice Dm is 14.98 g/kg, Dn is 2.30 g/kg;French Pinellia and Caowu decoction LD50 is 12.20 g/kg, the confidence interval is 11.36 to 13.10 g/kg. The LD50 of Pinellia ternata and Caowu decoction was 4.39 g/kg, the confidence interval was 3.69 to 5.28 g/kg;the LD50 of Herba decoction was 7.97 g/kg, and the confidence interval was 6.97 to 9.09 g/kg. Compared with the use of sylvestris alone, the LD50 of the genus Pseudostellaria sinensis was larger, and the LD50 of the genus Prunus chinensis was smaller. There were 13 chemical components in the two groups, and the Pinellia ternata and the Phyllostachys pubescens Internal, peak 13(deoxyaconitine), peak 9(northrazine), peak 12(10-deacetylated aconitine), peak 11(hypaconitine) and peak 10(new aconitine) Is a diester type alkaloid;peak 8(benzoyl aconitine), peak 6(benzoyl aconitine), peak 4(10-OH-benzoyl aconitine) is the monoester alkaloids increased significantly;the peaks 3(acktlin) and peak 1(alcobamine in the alkalamine alkaloids) increased not significantly, peak 2(neoolin) decreased significantly, and the other 2 unknown components increased not obvious. Conclusion:Compared with the herbicide alone, the toxicity of Pinellia ternata and D. chinensis decreased, and the toxicity of P. sylvestris and P. sylvestris increased. The toxicity of P. sylvestre and P. sinensis or P. sinensis increased. Or reduce the association with the ester alkaloid content.
作者
王璐
刘然
邢爽
张怡
许保海
WANG Lu;LIU Ran;XING Shuang(Dispensary of Traditional Chinese Medicine,Beijing Jishuitan Hospital,Beijing 100035,China)
出处
《四川中医》
2020年第1期58-62,共5页
Journal of Sichuan of Traditional Chinese Medicine
基金
国家自然科学基金项目(编号:81473581)
关键词
法半夏
生半夏
草乌
中药配伍
急性毒性
毒性成分
Rhizoma Pinellinae Praeparata
Born Pinellia ternata
Radix aconiti agrestis
Chinese medicine compatibility
Acute toxicity
Toxic components
