摘要
目的构建结直肠癌RNA调控网络,探讨结直肠癌中分子调控机制。方法从TCGA数据库中提取结直肠癌转录水平表达数据,筛选差异表达RNAs,结合miRDB,miRcode,miRTarBase和TargetScan数据库靶基因预测结果,整合LncRNA-miRNA、miRNA-mRNA关系对,基于ceRNA假说及LncRNA细胞内定位,筛选出胞质表达并介导该网络调控的LncRNA,重建结直肠癌中lncRNA-miRNA-mRNA作用网络。结果在结直肠癌差异表达基因分析中初步筛选出119个LncRNA、32个miRNA以及59个mRNA;识别777对LncRNA-miRNA与77对miRNA-mRNA,基于ceRNA假说以及LncRNA在细胞内定位,最后筛选出15个上调LncRNA、3个下调miRNA(hsa-mir-145,hsa-mir-193b,hsa-mir-150)以及5个上调mRNA重建ceRNA网络。结论成功构建结直肠癌中LncRNA介导调控的ceRNA网络,识别出预后相关的潜在靶点,为结直肠癌治疗研究提供了新的参考依据。
Objective To construct a RNA regulatory network for colorectal cancer and explore the molecular regulation mechanism of colorectal cancer.Methods The transcriptional level expression data of colorectal cancer were extracted from TCGA database,differentially expressed RNAs was selected.According to target prediction databases,miRDB,miRcode,miRTarBase and TargetScan,RNA pairs of Lnc-RNA-miRNA and miRNA-mRNA were identified.Basing on ceRNA hypothesis,and analysis of LncRNA intracellular localization,the cytoplasmic expression LncRNAs were identified to regulate the network.Finally the lnRNA-miRNA-mRNA in colorectal cancer was reconstructed.Results A total of 119 LncRNA,32 miRNAs and 59 mRNAs were screened from differentially expressed RNAs in colorectal cancer.777 pairs of LncRNA-miRNA and 77 pairs of miRNA-mRNA were identified.Based on the ceRNA hypothesis and cytoplasm expressed LncRNAs,15 up-regulated LncRNAs,3 down-regulated miRNAs(hsa-mir-145,hsa-mir-193 b,hsa-mir-150)and 5 up-regulated mRNAs were selected to reconstruct the ceRNA network.Conclusion The RNA network mediated by LncRNA in colorectal cancer was successfully constructed,and the potential targets were identified,which provided a reference for the treatment of colorectal cancer.
作者
邓巧玲
柳家翠
喻明霞
李胜
DENG Qiaoling;LIU Jiacui;YU Mingxia;LI Sheng(Department of Clinical Laboratory,Zhongnan Hospital,Wuhan University,Wuhan 430071,China;BioBank,Zhongnan Hospital of Wuhan,Wuhan 430071,China)
出处
《西部医学》
2020年第2期166-170,共5页
Medical Journal of West China
基金
国家自然科学基金(81472033
30901308)
湖北省自然科学基金(2013CFB233)
武汉大学珞珈青年学者科研基金(351人才计划)
武汉大学学位与研究生教育教学改革研究项目(2018)
2018年武汉大学医学部教学改革研究项目(2018058)
