摘要
为掌握江西省猪伪狂犬病病毒(PRV)的分子流行病学及遗传变异情况,本研究运用实验室已建立的PCR方法对2013~2018年采集自江西南昌、宜春、赣州、吉安、九江、上饶、抚州、新余8个地区的PRV阳性猪病料进行gE和gB基因扩增,并对PCR产物进行测序和序列分析。结果显示,共获得64株PRV的gE基因序列和12株PRV的gB基因序列;gE基因遗传进化树表明,64株PRV同属一个大分支,与亚洲毒株及近年来国内分离株亲缘关系较近,全部为GⅠ型,而与GⅡ型的欧美经典毒株亲缘关系较远;江西毒株gE基因与19株参考毒株的核苷酸和氨基酸序列同源性分别为97.2%~100%和94.6%~100%;gB基因与11株参考毒株的核苷酸和氨基酸序列同源性分别为98.2%~100%和96.5%~100%;相较于Bartha-K61疫苗株,江西流行毒株的gB基因存在碱基缺失、插入和位点突变现象。本研究从分子流行病学角度证实了江西省PRV的流行与变异情况,为江西省科学防控PRV提供理论依据。
In order to grasp the molecular epidemiology and genetic variation of pseudorabies virus(PRV)in Jiangxi province,this study used the established PCR method in the laboratory to amplify gE and gB genes of the collected PRV positive tissue samples from Nanchang,Yichun,Ganzhou,Ji’an,Jiujiang,Shangrao,Fuzhou and Xinyu in Jiangxi province during 2013 to 2018,and the PCR products were sequenced and analyzed.The results showed that a total of 64 PRV gE gene sequences and 12 PRV gB gene sequences were obtained.The phylogenetic tree of gE gene showed that 64 strains of PRV belonged to one large branch,and they were closely related to Asian strains and domestic isolates in recent years,all of which were GⅠtype,but had far-reaching relationship with GⅡtype European and American classic strains;The nucleotide and amino acid homology bewteen Jiangxi strain gE gene and 19 reference strains were 97.2%to 100%and 94.6%to 100%,respectively;The nucleotide and amino acid homologies between gB gene and 11 reference strains were 98.2%to 100%and 96.5%to 100%,respectively;There were base deletions,insertion and site mutations exist in gB gene of Jiangxi strain compared with Bartha-K61 vaccine strain.This study confirmed the prevalence and variation of PRV in Jiangxi province from the perspective of molecular epidemiology,and provided theoretical basis for scientific prevention and control of PRV in Jiangxi province.
作者
高映雪
蒋新华
周荷田
罗锋
杨丹凤
万文忠
邓舜洲
GAO Yingxue;JIANG Xinhua;ZHOU Hetian;LUO Feng;YANG Danfeng;WAN Wenzhong;DENG Shunzhou(Jiangxi Agricultural University,Nanchang 330045,China;Jiangxi JinYibo Biotechnology Company,Nanchang 330013,China;Jiangxi Animal Disease Control Center,Nanchang 330096,China)
出处
《中国畜牧兽医》
CAS
北大核心
2020年第1期164-173,共10页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31460666)
江西省科技支撑计划项目(20132BBF60044、20141BBF60039)
江西省现代农业产业技术体系建设专项资金(JXARS-03)
