摘要
Both of carbon dioxide(CO2)and near-infrared(NIR)light as triggers for non-invasive remotely control are attracting wide attentions due to their good biocompatibility and easy operation.Here,CO2/NIR light dual controlled nanoparticles are proposed to remotely regulate the unzipping of dsDNA by using imidazole functionalized conjugated polymer nanoparticles(imidazole-CPNs).The dsDNA successfully coats on the shell of imidazole-CPNs to form imidazole-CPNs/dsDNA assembly due to intensively electrostatic interaction triggered by CO2.Furthermore,the unzipping process of dsDNA is remotely controlled by NIR light based on the photothermal effect,and it can be readily monitored by the fluorescence intensity of ethidium bromide(EB)and CD spectra of dsDNA.Thus,dual stimulation responsive imidazole-CPNs effectively control dsDNA unzipping under CO2 stimulus and NIR light,promising a new direction in the biological applications of DNA,such as the treatments of diseases caused by gene duplication abnormality.
Both of carbon dioxide（CO2） and near-infrared（NIR） light as triggers for non-invasive remotely control are attracting wide attentions due to their good biocompatibility and easy operation.Here,CO2/NIR light dual controlled nanoparticles are proposed to remotely regulate the unzipping of dsDNA by using imidazole functionalized conjugated polymer nanoparticles（imidazole-CPNs）.The dsDNA successfully coats on the shell of imidazole-CPNs to form imidazole-CPNs/dsDNA assembly due to intensively electrostatic interaction triggered by CO2.Furthermore,the unzipping process of dsDNA is remotely controlled by NIR light based on the photothermal effect,and it can be readily monitored by the fluorescence intensity of ethidium bromide（EB） and CD spectra of dsDNA.Thus,dual stimulation responsive imidazole-CPNs effectively control dsDNA unzipping under CO2 stimulus and NIR light,promising a new direction in the biological applications of DNA,such as the treatments of diseases caused by gene duplication abnormality.
基金
financial support of the National Natural Science Foundation of China (Nos.21574037,21773054 and 51803046)
the "100 Talents" Program of Hebei Province,China (No.E2014100004)
the Natural Science Foundation of Hebei Province (No.B2017202051)
the Program for Top 100 Innovative Talents in Colleges and Universities of Hebei Province (No. SLRC2017028)
