摘要
为探讨添加不同浓度外源一氧化氮(Nitric oxide,NO)对小麦盐害的缓解作用,确定最适添加浓度,本研究以山农22号为供试小麦品种,以硝普钠(Sodium-nitroprusside,SNP)为外源NO供体,采用液培方式,研究不同浓度SNP(0、25、50、100、150、200μmol L^-1)对100 mmol L^-1NaCl胁迫下小麦幼苗生长及生理特性的影响。结果表明,100 mmol L^-1NaCl显著抑制了小麦幼苗的生长,添加不同浓度SNP均能缓解盐胁迫对小麦幼苗生长的抑制作用,提高小麦幼苗体内抗坏血酸(Ascorbic acid,As A)含量和亚细胞各组分中超氧化物歧化酶(Superoxide dismutase,SOD)活性,减少丙二醛(Malondialdehyde,MDA)和过氧化氢(Hydrogen peroxide,H2O2)的积累,减轻膜脂过氧化毒害;促进脯氨酸的合成与积累,降低电解质渗透率;抑制小麦对Na的吸收,增加对其它必需矿质养分的吸收;提高小麦幼苗叶片的叶绿素含量,促进光合作用;增加小麦幼苗的干鲜重,促进小麦生长。不同浓度SNP对小麦幼苗盐胁迫的缓解作用随SNP浓度的增加先升高后降低,以100μmol L^-1SNP对小麦幼苗盐胁迫的缓解作用最为明显。
The purpose of this study was to investigate the mitigation effect of adding different concentrations of exogenous nitric oxide(NO)on wheat salt damage and to determine an optimum concentration of NO.Sodium nitroprusside(SNP)was used as the exogenous NO donor and the effects of different concentrations of SNP(0,25,50,100,150,200μmol L^-1)on the growth and physiological characteristics of wheat seedlings(Shannong 22)were analyzed under salt stress of 100 mmol L^-1 Na Cl by solution culture.The results showed that the salt stress of 100 mmol L^-1 Na Cl significantly inhibited the growth of wheat seedlings.The addition of different concentrations of SNP alleviated the salt stress,increased the superoxide dismutase(SOD)activities of subcellular components in wheat seedlings and the ascorbic acid content,reduced the accumulation of malondialdehyde(MDA)and hydrogen peroxide(H2O2)and the lipid peroxidation toxicity,promoted the synthesis and accumulation of proline and reduced the electrolyte permeability.The absorption of Na by wheat was inhibited and the absorption of other essential mineral nutrients by wheat was increased under the salt stress of 100 mmol L^-1 NaCl.Also,the salt stress of 100 mmol L^-1 Na Cl increased the photosynthetic pigment contents of wheat seedling leaves and the dry and fresh weights of wheat seedlings,and promoted the photosynthesis and wheat growth.The mitigation effect of different concentrations of SNP on wheat salt damage increased first and then decreased with the increase of SNP concentration,and was the most significant under 100μmol L^-1 SNP.
作者
王茂莹
王慧桥
司庆臣
张倩
朱营营
董元杰
WANG Mao-ying;WANG Hui-qiao;SI Qing-chen;ZHANG Qian;ZHU Ying-ying;DONG Yuan-jie(College of Resources and Environment,Shandong A gric ultural University,Tai'an 271018,China;Qihe County Agricultural and Aural Bureau,Qi'he 251100,China)
出处
《土壤通报》
CAS
CSCD
北大核心
2019年第6期1426-1433,共8页
Chinese Journal of Soil Science
基金
山东省重大科技创新工程项目（2017CXGC0301）
国家重点研发计划项目（2017YFD0201705）
山东省农业重大应用技术创新项目（SD2019ZZ021）资助
关键词
外源一氧化氮
硝普钠
盐胁迫
小麦幼苗
生理特性
最适浓度
Exogenous NO
Sodium nitroprusside
Salt stress
Wheat seedling
Physiological characteristics
Optimum concentration
