摘要
试验旨在研究卵钙蛋白-32(ovocalyxin-32,OCX-32)、卵清蛋白(ovalbumin,OVA)基因多态性与蛋品质性状的关联性,寻找与蛋鸡蛋品质性状相关的分子遗传标记。采用PCR-SSCP技术对海兰褐蛋鸡父母代中OCX-32、OVA基因的多态位点进行检测,使用SPSS 22.0软件将不同基因型与海兰褐蛋鸡蛋品质性状进行关联分析,并进一步在子代中验证,寻找优势基因型。结果显示,OCX-32基因第6外显子中检测到AA、BB和CC 3种基因型,2个突变位点,分别位于7018和7116 bp;OVA基因第5外显子中检测到AA、BC、BB 3种基因型,2个突变位点位于4296和4323 bp。关联分析结果表明,海兰褐蛋鸡父母代OCX-32基因AA基因型的蛋黄重极显著高于BB和CC基因型(P<0.01);海兰褐蛋鸡父母代OVA基因AA基因型的哈氏单位及蛋白高度极显著高于BC基因型(P<0.01),显著高于BB基因型(P<0.05),AA基因型蛋白重显著高于BC基因型(P<0.05)。在子代中验证进一步确定了OCX-32基因的AA基因型为调控蛋黄重的优势基因型,OVA基因的AA基因型为调控哈氏单位、蛋白高度和蛋白重的优势基因型。综上所述,OCX-32及OVA基因对蛋品质性状有一定影响,可作为蛋鸡的分子育种标记。
This study was aimed to investigate the association between ovocalyxin-32(OCX-32)and ovalbumin(OVA)genes polymorphism and egg quality traits,and find the molecular genetic markers associated with egg quality traits in laying hens.PCR-SSCP method was used to detect the polymorphism of OCX-32 and OVA genes in the parents’generation of Hyline Brown laying hens.The correlation between different genotypes and the egg quality traits was analyzed by SPSS 22.0 software,and further verified in the offspring to find the dominant genotype.The results showed that three genotypes(AA,BB and CC),two mutation sites located at 7018 and 7116 bp were detected in exon 6 of OCX-32 gene,and three genotypes(AA,BC and BB),two mutation sites located at 4296 and 4323 bp were detected in exon 5 of OVA gene.The results of association analysis showed that the egg yolk weight of OCX-32 gene AA genotype of parents was extremely significantly higher than BB and CC genotypes(P<0.01).The Haugh unit and protein height of OVA gene AA genotype of parents were extremely significantly higher than BC genotype(P<0.01),which was significantly higher than BB genotype(P<0.05),the albumen weight of AA genotype was significantly higher than BC genotype(P<0.05).Verification in the offspring confirmed that AA genotype of OCX-32 gene was the dominant genotype for regulating egg yolk weight,and AA genotype of OVA gene was the dominant genotype for regulating Haugh unit,protein height and protein weight.In summary,OCX-32 and OVA genes had certain effects on egg quality traits,which could be used as molecular breeding markers for laying hens.
作者
张蕾
任嵩
张璐
孙杰
ZHANG Lei;REN Song;ZHANG Lu;SUN Jie(College of Animal Science and Technology,Shihezi University,Shihezi 832000,China)
出处
《中国畜牧兽医》
CAS
北大核心
2020年第2期514-523,共10页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(31660654)
