摘要
使用离体培养法检测不同玉米材料花粉萌发率并于显微镜下观察花粉粒直径,研究转Cry1Ab、EPSPS基因玉米对花粉生活力的影响。结果表明,刚离体花粉萌发活力最强,培养180 min后花粉萌发率可达89.4%,高温(36℃)条件下放置2 h花粉基本丧失萌发能力。与非转基因对照相比,转Cry1Ab、EPSPS基因玉米对花粉离体后的生存能力无显著影响,对花粉粒直径大小无影响。
In order to determine the effect of Cry1Ab and EPSPS gene maize on the pollen viability, the pollen germination rate of different maize materials was detected by the method of vitro culture and the diameter of pollen grains was observed under the microscope. The results showed that pollen germination rate of maize was the strongest when the pollen just in vitro which approximately 89.4% after 180 min. The pollen was almost completely inactivated when put it at high temperature(36℃) for 120 minutes. No significant effect on the viability of pollen in vitro and the diameter of pollen grains between the transgenic maize and the normal control.
作者
刘来盘
刘标
LIU Lai-pan;LIU Biao(College of Life Science,Nanjing University,Nanjing 210023;Nanjing Institute of Environmental Sciences,Ministry of Ecology and Environment,Nanjing 210042,China)
出处
《玉米科学》
CAS
CSCD
北大核心
2019年第6期58-61,共4页
Journal of Maize Sciences
基金
转基因生物新品种培育重大专项“自然生态风险监测与控制技术”重大课题项目(2016ZX08012-005)
抗虫抗除草剂转基因玉米“C0030.3.5”产业化研究(2015ZX08013002)
